AB3935

Anti-UBE2C 抗体

Name: Anti-UBE2C 抗体 (ab3935) | Abcam
Brand: Abcam Limited
SKU: AB3935
Price: 76000 JPY
Availability: InStock

Anti-UBE2C antibody

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(3 Publications)

Goat Polyclonal UBE2C antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human UBE2C aa 150 to C-terminus.

別名を表示する

UBCH10, UBE2C, Ubiquitin-conjugating enzyme E2 C, (E3-independent) E2 ubiquitin-conjugating enzyme C, E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C

2 Images

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Immunoprecipitation - Anti-UBE2C antibody (AB3935)

ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (10⁶ cells/ml) using 40μl of ab3935 saturated protein G dynabeads, by Western blot (ECL) :

Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
Lane 4 = Noc arrested HeLa whole cell lysate.

For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

All lanes:

Immunoprecipitation - Anti-UBE2C antibody (ab3935)

Predicted band size: 20 kDa

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Western blot - Anti-UBE2C antibody (AB3935)

ab3935 at a 2.5μg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL) :

Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2μl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2μl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20μl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20μl).

For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL) :

Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

For a more detailed descri

All lanes:

Western blot - Anti-UBE2C antibody (ab3935)

Predicted band size: 20 kDa

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Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human UBE2C aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>(See Abreview)</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UBE2C also known as ubiquitin-conjugating enzyme E2C or UBCH10 is an enzyme that plays a role in the ubiquitination process. Its molecular mass is approximately 20 kDa. UBE2C is an E2 ubiquitin-conjugating enzyme actively involved in marking proteins for degradation through the ubiquitin-proteasome system. It facilitates the transfer of ubiquitin to target proteins cooperating with E3 ligases. UBE2C shows expression in various tissues with high levels observed in proliferating cells such as those found in tumors.

Biological function summary

UBE2C is involved in cell cycle regulation by targeting specific proteins for ubiquitination and degradation. It collaborates with the anaphase-promoting complex/cyclosome (APC/C) an important E3 ligase complex ensuring the proper progression of the cell cycle especially during the metaphase-anaphase transition. Through this action UBE2C aids in maintaining genomic stability by preventing the accumulation of proteins that can interfere with cell division.

Pathways

The role of UBE2C is integral in the cell cycle and ubiquitin-proteasome pathways. The enzyme participates in the proper execution of the metaphase-anaphase transition and degradation of cell cycle regulators like cyclin B. UBE2C interacts with proteins such as CDC20 a co-activator of APC/C to ensure accurate coordination of cell division events. Its function in the ubiquitin-proteasome pathway emphasizes its role in maintaining protein homeostasis.

UBE2C's dysfunction or overexpression associates with cancer development and progression. Overexpression of UBE2C has been observed in various cancers including breast cancer and prostate cancer and may contribute to tumorigenesis by disrupting cell cycle control. UBE2C can interact with oncoproteins such as MYC further linking its dysregulation to cancer pathways. Understanding the relationships between UBE2C and cancer may offer insights into therapeutic targets for tumor treatment.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

See full target information UBE2C

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 33:2529-36 PubMed25760688

2015

Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingyi Zhang,Ting Han,Guo Wei,Yajie Wang

Gynecologic oncology 131:368-73 PubMed23911795

2013

Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Peter A Argenta,Inhwa Um,Charlene Kay,David Harrison,Dana Faratian,Thanasak Sueblinvong,Melissa A Geller,Simon P Langdon

International journal of cancer 121:33-8 PubMed17354233

2007

Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin-conjugating enzyme E2C gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Ieta,Eiki Ojima,Fumiaki Tanaka,Yoshito Nakamura,Naotsugu Haraguchi,Koshi Mimori,Hiroshi Inoue,Hiroyuki Kuwano,Masaki Mori

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-UBE2C 抗体

Anti-UBE2C antibody

Immunoprecipitation - Anti-UBE2C antibody (AB3935)

Western blot - Anti-UBE2C antibody (AB3935)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise