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AB108929

Anti-Ube1L 抗体 [EPR4270]

Anti-Ube1L antibody [EPR4270]

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Rabbit Recombinant Monoclonal Ube1L antibody. Suitable for WB, ICC/IF and reacts with Human samples.

別名を表示する

UBE1L, UBE2, UBA7, Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog

3 Images
Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)
  • WB

Unknown

Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)

All lanes:

Western blot - Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HL60 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Lane 5:

Fetal liver lysate at 10 µg

Predicted band size: 111 kDa

false

Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)
  • WB

Lab

Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution

Lane 1:

GST tagged Recombinant human UBA7(Ube1L) protein (Full length, 166 KDa)

Lane 2:

GST tagged Recombinant human UBA1 protein (Full length, 146 KDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 111 kDa

Observed band size: 146 kDa,166 kDa

false

Exposure time: 180s

Immunocytochemistry/ Immunofluorescence - Anti-Ube1L antibody [EPR4270] (AB108929)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ube1L antibody [EPR4270] (AB108929)

Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Ube1L antibody [EPR4270] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4270

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ube1L also known as ubiquitin-activating enzyme E1-like plays an important role in the ubiquitin-proteasome system by activating ubiquitin molecules. With a molecular mass of approximately 117 kDa Ube1L functions as the initial enzyme in the ubiquitination cascade catalyzing the formation of a thioester bond with ubiquitin. This process is important for the tagging of proteins for degradation. Ube1L exhibits widespread expression in various tissues indicating its importance in diverse cellular activities.
Biological function summary

This protein participates actively in the regulation of protein homeostasis. Ube1L helps to manage protein turnover and maintains protein quality control by marking damaged or unneeded proteins for degradation via the proteasome. Although Ube1L functions primarily as a standalone enzyme it cooperates closely with ubiquitin-conjugating and -ligating enzymes (E2s and E3s) to achieve precise ubiquitination of target proteins. This cooperation is critical for cellular processes like cell cycle regulation and response to stress.

Pathways

Ube1L is a significant element in the ubiquitin-proteasome pathway which is essential for protein degradation and turnover. It also contributes to the regulation of interferon signaling by modifying proteins such as ISG15 a ubiquitin-like protein involved in antiviral response. Related proteins such as UBE2L6 and UBE2E1 serve as E2 conjugating enzymes that interact with Ube1L further influencing these pathways by facilitating the transfer of activated ubiquitin.

Ube1L has been implicated in certain types of cancers and viral infections. Alterations in the expression or function of Ube1L can lead to disrupted protein degradation contributing to cancer progression through the accumulation of oncogenic proteins. Ube1L is also connected to proteins like ISG15 and USP18 which are involved in host defense mechanisms against viral infections. Dysregulation in these contexts can result in inadequate antiviral responses or improper regulation of cell proliferation.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation (PubMed : 27545325). Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus (PubMed : 16254333, PubMed : 19073728, PubMed : 29056542, PubMed : 29743376, PubMed : 37722521). For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition (PubMed : 20133869). ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling (PubMed : 27564865).
See full target information UBA7
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB133479

Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)]

primary-antibodies

ube1l-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr42692-ab133479

4

(2 reviews)

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