Anti-UBAP2L 抗体 (ab70319)
Key features and details
- Rabbit polyclonal to UBAP2L
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-UBAP2L antibody
UBAP2L 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UBAP2L -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region between residues 400 and 450 of human UBAP2L (AAH03170.1).
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ポジティブ･コントロール
- WB: HeLa whole cell lysate; IP: HEK293T cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70319の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/25000. Detects a band of approximately 160 kDa (predicted molecular weight: 115 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/10000 - 1/25000. Detects a band of approximately 160 kDa (predicted molecular weight: 115 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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組織特異性
Ubiquitous. -
配列類似性
Contains 1 UBA domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Acetylated. - Information by UniProt
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参照データベース
- Entrez Gene: 9898 Human
- Omim: 616472 Human
- SwissProt: Q14157 Human
- Unigene: 490551 Human
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別名
- FLJ42300 antibody
- KIAA0144 antibody
- NICE 4 antibody
see all
画像
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Western blot - Anti-UBAP2L antibody (ab70319)All lanes : Anti-UBAP2L antibody (ab70319) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 115 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-UBAP2L antibody (ab70319)UBAP2L was immunoprecipitated from HEK-293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70319 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70319 at 0.04 µg/mL.
Lane 1: ab70319 IP in HEK-293T whole cell lysate.
Lane 2: ab70319 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab70319 は 8 報の論文で使用されています。
- Jia X et al. De novo variants in genes regulating stress granule assembly associate with neurodevelopmental disorders. Sci Adv 8:eabo7112 (2022). PubMed: 35977029
- Wang Z et al. Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation. Mol Cancer 21:92 (2022). PubMed: 35366893
- Huang R et al. Norcantharidin-blocked ANXA2P2 inhibits fibroblast proliferation by increasing UBAP2L mRNA stability through LIN28B. Life Sci 279:119645 (2021). PubMed: 34043991
- Pan Y et al. MicroRNA-19a-3p inhibits the cellular proliferation and invasion of non-small cell lung cancer by downregulating UBAP2L. Exp Ther Med 20:2252-2261 (2020). PubMed: 32765702
- He J et al. UBAP2L silencing inhibits cell proliferation and G2/M phase transition in breast cancer. Breast Cancer 25:224-232 (2018). WB ; Human . PubMed: 29196913
- Lin GL et al. Upregulation of UBAP2L in Bone Marrow Mesenchymal Stem Cells Promotes Functional Recovery in Rats with Spinal Cord Injury. Curr Med Sci 38:1081-1089 (2018). PubMed: 30536073
- Zhao B et al. Downregulation of ubiquitin-associated protein 2-like with a short hairpin RNA inhibits human glioma cell growth in vitro. Int J Mol Med 36:1012-8 (2015). WB . PubMed: 26310274
- Li D & Huang Y Knockdown of ubiquitin associated protein 2-like inhibits the growth and migration of prostate cancer cells. Oncol Rep 32:1578-84 (2014). PubMed: 25069639