Anti-Uba6 抗体 (ab177514)
Key features and details
- Rabbit polyclonal to Uba6
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Uba6 antibody
Uba6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Uba6 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chicken, Cow, Pig, Chimpanzee, Gorilla, Orangutan, Zebra finch
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免疫原
Synthetic peptide within Human Uba6 aa 200-250. The exact sequence is proprietary. NP_060697.4
Sequence:GDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFRE I
Database link: A0AVT1 -
ポジティブ･コントロール
- Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1 and NIH3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177514の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 118 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 118 kDa. |
ターゲット情報
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機能
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility. -
組織特異性
Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-activating E1 family. - Information by UniProt
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参照データベース
- Entrez Gene: 422655 Chicken
- Entrez Gene: 461294 Chimpanzee
- Entrez Gene: 518834 Cow
- Entrez Gene: 101125792 Gorilla
- Entrez Gene: 55236 Human
- Entrez Gene: 231380 Mouse
- Entrez Gene: 100433991 Orangutan
- Entrez Gene: 100512547 Pig
see all -
別名
- E1-L2 antibody
- FLJ10808 antibody
- FLJ23367 antibody
see all
画像
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Western blot - Anti-Uba6 antibody (ab177514)All lanes : Anti-Uba6 antibody (ab177514) at 0.4 µg/ml
Lane 1 : Whole cell lysate from 293T cells
Lane 2 : Whole cell lysate from HeLa cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from TCMK-1 cells
Lane 5 : Whole cell lysate from NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 118 kDa
Exposure time: 10 seconds
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab177514 は論文での使用が確認できていません。