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AB109230

Anti-UBA52 抗体 [EPR4547]

Anti-UBA52 antibody [EPR4547]

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(1 Publication)

Rabbit Recombinant Monoclonal UBA52 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

UBCEP2, UBA52, Ubiquitin-ribosomal protein eL40 fusion protein, CEP52, Ubiquitin A-52 residue ribosomal protein fusion product 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (AB109230)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (AB109230)

ab109230 stained HepG2 cells. The cells were 4% formaldehdye fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109230 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight 488 goat anti-rabbit IgG used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Western blot - Anti-UBA52 antibody [EPR4547] (AB109230)
  • WB

Unknown

Western blot - Anti-UBA52 antibody [EPR4547] (AB109230)

All lanes:

Western blot - Anti-UBA52 antibody [EPR4547] (ab109230) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

Fetal kidney lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 10 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (AB109230)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (AB109230)

Immunohistochemical analysis of UBA52 in paraffin embedded Human brain tissue using ab109230 at a 1/500 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-UBA52 antibody [EPR4547] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4547

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p>Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UBA52 also known as Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 is a protein fusion of ubiquitin and ribosomal protein L40. It has a mass of approximately 15 kDa. UBA52 expresses widely in various tissues including the liver brain and heart playing a role in protein synthesis and ubiquitination. The ubiquitin domain of UBA52 gets translated and cleaved to participate in the ubiquitin-proteasome system while the ribosomal component integrates into the 60S subunit of the ribosome.
Biological function summary

UBA52 functions as both a ribosomal protein and a source of ubiquitin. This dual role enables it to participate in protein synthesis as part of the ribosome. Additionally it provides ubiquitin required for post-translational modification of proteins affecting protein stability and degradation. UBA52 is part of the ribosome complex and contributes to efficient protein translation while its ubiquitin segment influences protein turnover and cellular response to stress.

Pathways

UBA52 plays roles in protein translation and ubiquitin-mediated proteolysis. These pathways are essential for maintaining cellular homeostasis. In the context of protein synthesis UBA52 associates with other ribosomal proteins and supports the assembly of the ribosome facilitating mRNA translation. In the ubiquitin-proteasome pathway UBA52 contributes ubiquitin moieties used to tag proteins for degradation working with E2 and E3 ligases such as UBE2K and HUWE1 important for regulated proteolysis.

UBA52 is linked to conditions involving protein synthesis defects and improper protein degradation including cancer and neurodegenerative disorders. Changes in UBA52 expression or mutations can disrupt ribosome function affecting cellular proliferation and survival in cancers. Additionally impaired ubiquitin signaling has implications in neurodegenerative diseases where protein aggregates form due to faulty degradation. UBA52's connection to pathways with proteins like p53 in cancer and Parkin in neurodegeneration further illustrates its relevance across different pathologies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.. Large ribosomal subunit protein eL40. Component of the 60S subunit of the ribosome (PubMed : 23169626, PubMed : 23636399, PubMed : 32669547, PubMed : 39048817, PubMed : 39103523). Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs (PubMed : 23169626, PubMed : 23636399, PubMed : 32669547, PubMed : 39048817, PubMed : 39103523).
See full target information UBA52
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:36780 PubMed27829658

2016

The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Kobayashi,Shigeru Oshima,Chiaki Maeyashiki,Yoichi Nibe,Kana Otsubo,Yu Matsuzawa,Yasuhiro Nemoto,Takashi Nagaishi,Ryuichi Okamoto,Kiichiro Tsuchiya,Tetsuya Nakamura,Mamoru Watanabe
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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