Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-UAP56 抗体 [EPR13144-62] (ab181061)

Submit a review Submit a question References (2)
Western blot - Anti-UAP56 antibody [EPR13144-62] (ab181061)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP56 antibody [EPR13144-62] (ab181061)
  • Immunocytochemistry/ Immunofluorescence - Anti-UAP56 antibody [EPR13144-62] (ab181061)
  • Flow Cytometry (Intracellular) - Anti-UAP56 antibody [EPR13144-62] (ab181061)
  • Anti-UAP56 antibody [EPR13144-62] (ab181061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13144-62] to UAP56
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-UAP56 antibody [EPR13144-62]
    UAP56 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13144-62] to UAP56

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562, HeLa, Jurkat and 293 cell lysate. Human infiltrating duct breast carcinoma tissue. HeLa and Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181061の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

ターゲット情報

  • 機能

    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.

  • 配列類似性

    Belongs to the DEAD box helicase family. DECD subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.

  • ドメイン

    The helicase C-terminal domain mediates interaction with THOC4.

  • 細胞内局在

    Nucleus. Nucleus speckle.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 0610030D10Rik antibody
    • 4F2-LC6 antibody
    • 56 kDa U2AF65-associated protein antibody
    • AI428441 antibody
    • ATP-dependent RNA helicase p47 antibody
    • B(0,+)-type amino acid transporter 1 antibody
    • BAT1 antibody
    • Bat1a antibody
    • D17H6S81E antibody
    • D17H6S81E-1 antibody
    • D6S81E antibody
    • D6S81Eh antibody
    • DDX39B antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B antibody
    • DEAD box protein UAP56 antibody
    • DX39B_HUMAN antibody
    • EC 3.6.1.- antibody
    • Glycoprotein-associated amino acid transporter b0,+AT1 antibody
    • HLA-B-associated transcript 1 protein antibody
    • HLA-B-associated transcript 1A antibody
    • HLA-B-associated transcript-1 antibody
    • MGC127051 antibody
    • MGC19235 antibody
    • MGC38799 antibody
    • nuclear RNA helicase (DEAD family) antibody
    • OTTHUMP00000029229 antibody
    • OTTHUMP00000165889 antibody
    • OTTHUMP00000165890 antibody
    • p47 antibody
    • Solute carrier family 7 member 9 antibody
    • Spliceosome RNA helicase BAT1 antibody
    • Spliceosome RNA helicase DDX39B antibody
    • U2AF65-associayed protein, 56-KD antibody
    • UAP56 antibody
    see all

画像

  • Western blot - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Western blot - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    All lanes : Anti-UAP56 antibody [EPR13144-62] (ab181061) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP56 antibody [EPR13144-62] (ab181061)

    Immunohistochemical analysis of paraffin embedded Human infiltrating duct breast carcinoma tissue sections labeling UAP56 using ab181061 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Immunocytochemistry/ Immunofluorescence - Anti-UAP56 antibody [EPR13144-62] (ab181061)

    Immunofluorescent analysis of acetone fixed HeLa cells labeling UAP56 using ab181061 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Flow Cytometry (Intracellular) - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Flow Cytometry (Intracellular) - Anti-UAP56 antibody [EPR13144-62] (ab181061)

    Intracellular Flow Cytometry analysis of Jurkat cells labeling UAP56 using ab181061 at a 1/10 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde. 

     

     

  • Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Anti-UAP56 antibody [EPR13144-62] (ab181061)

参考文献 (2)

ab181061 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181061 は 2 報の論文で使用されています。

  • Awasthi S  et al. DDX49 is an RNA helicase that affects translation by regulating mRNA export and the levels of pre-ribosomal RNA. Nucleic Acids Res 46:6304-6317 (2018). PubMed: 29618122
  • Awasthi S  et al. DDX39B promotes translation through regulation of pre-ribosomal RNA levels. RNA Biol 15:1157-1166 (2018). PubMed: 30176153

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab181061.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com