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AB95949

Anti-UAP1 抗体

Anti-UAP1 antibody

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(4 Publications)

Rabbit Polyclonal UAP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UAP1 aa 50-500.

別名を表示する

SPAG2, UAP1, UDP-N-acetylhexosamine pyrophosphorylase, Antigen X, Protein-pyrophosphorylation enzyme, Sperm-associated antigen 2, UDP-N-acetylgalactosamine pyrophosphorylase, UDP-N-acetylglucosamine pyrophosphorylase, AGX

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-UAP1 antibody (AB95949)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UAP1 antibody (AB95949)

Immunofluorescence analysis of paraformaldehyde fixed A549 cells using ab95949 : (1) at 1/200 dilution, (2) merged with DNA probe.

Western blot - Anti-UAP1 antibody (AB95949)
  • WB

Unknown

Western blot - Anti-UAP1 antibody (AB95949)

gel concentration : 7.5%

All lanes:

Western blot - Anti-UAP1 antibody (ab95949) at 1/3000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody (AB95949)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody (AB95949)

Immunohistochemical analysis of paraffin embedded ES 2 xenograft using ab95949 at 1/500 dilution.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human UAP1 aa 50-500. The exact immunogen used to generate this antibody is proprietary information.

Q16222

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

UDP-N-acetylglucosamine pyrophosphorylase also known as UAP1 is an enzyme that catalyzes the formation of uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) from UTP and N-acetylglucosamine-1-phosphate. UAP1 has a mass of approximately 56 kDa. This enzyme is expressed in various tissues but shows higher expression levels in skeletal muscle heart and liver. In these tissues UAP1 plays a significant role in synthesizing nucleotides and certain sugars that are essential for other cellular processes.
Biological function summary

The role of UAP1 is central to the biosynthetic pathway of complex carbohydrates. UAP1 participates in the synthesis of UDP-GlcNAc an essential precursor for glycoproteins and glycosaminoglycans. This function positions UAP1 as a component of a larger amino sugar complex involved in glycosylation which is critical for protein folding and stability. By producing UDP-GlcNAc UAP1 influences various cellular functions including signaling and metabolism.

Pathways

UAP1 lies at the intersection of the hexosamine biosynthetic pathway and glycosylation processes. It provides the substrate necessary for O-GlcNAcylation a modification that impacts protein function and regulation. This pathway links UAP1 to other proteins such as O-GlcNAc transferase (OGT) which modifies serine and threonine residues of many proteins. Together they affect cellular activities like nutrient sensing and transcription.

Altered UAP1 function links to metabolic and degenerative conditions. Abnormal UAP1 activity associates with type 2 diabetes due to its role in the hexosamine biosynthetic pathway that modulates insulin signaling. Furthermore its involvement in glycosylation pathways connects UAP1 to congenital disorders of glycosylation (CDG) where deficient glycosylation leads to multiorgan dysfunction. Changes in UAP1 expression or function can impair glycosylation potentially leading to dysregulation of related proteins such as insulin receptor substrates in diabetes or enzymes impacted in CDG.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the last step in biosynthesis of uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by converting UTP and glucosamine 1-phosphate (GlcNAc-1-P) to the sugar nucleotide (PubMed : 9603950, PubMed : 9765219). Also converts UTP and galactosamine 1-phosphate (GalNAc-1-P) into uridine diphosphate-N-acetylgalactosamine (UDP-GalNAc) (PubMed : 9765219). In addition to its role in metabolism, acts as a regulator of innate immunity in response to virus infection by mediating pyrophosphorylation of IRF3 : catalyzes pyrophosphorylation of IRF3 phosphorylated at 'Ser-386' by TBK1, promoting IRF3 dimerization and activation, leading to type I interferon responses (PubMed : 36603579).. Isoform AGX1. Isoform AGX1 has 2 to 3 times higher activity towards galactosamine 1-phosphate (GalNAc-1-P).. Isoform AGX1. Isoform AGX2 has 8 times more activity towards glucosamine 1-phosphate (GlcNAc-1-P).
See full target information UAP1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadq0334 PubMed40249802

2025

Targeting PGM3 abolishes SREBP-1 activation-hexosamine synthesis feedback regulation to effectively suppress brain tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Huali Su,Yaogang Zhong,Liqing He,Feng Geng,Xinmin Yin,Yongjun Kou,Cheng-Yao Chiang,Xiaokui Mo,Yunzhou Fan,Yanwei Liu,Qiang Wang,Shino Magaki,Timothy F Cloughesy,Etienne Lefai,William H Yong,Arnab Chakravarti,Xiang Zhang,Deliang Guo

Frontiers in immunology 13:841299 PubMed35479087

2022

Inhibition of O-GlcNAcylation Decreases the Cytotoxic Function of Natural Killer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Feinberg,Parameswaran Ramakrishnan,Derek P Wong,Abhishek Asthana,Reshmi Parameswaran

Molecular genetics and metabolism 128:444-451 PubMed31648943

2019

Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria.

Applications

Unspecified application

Species

Unspecified reactive species

Parith Wongkittichote,Gary Cunningham,Marshall L Summar,Elena Pumbo,Patrick Forny,Matthias R Baumgartner,Kimberly A Chapman

Cellular and molecular life sciences : CMLS 77:2605-2620 PubMed31560077

2019

Fbxl17 is rearranged in breast cancer and loss of its activity leads to increased global O-GlcNAcylation.

Applications

Unspecified application

Species

Unspecified reactive species

Bethany Mason,Susanne Flach,Felipe R Teixeira,Raquel Manzano Garcia,Oscar M Rueda,Jean E Abraham,Carlos Caldas,Paul A W Edwards,Heike Laman
View all publications
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組合せ製品

Primary Antibodies

AB128094

Anti-PGM3 antibody [OTI1A11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGM3 antibody [OTI1A11] (AB128094)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB2739

Anti-O-Linked N-Acetylglucosamine antibody [RL2]

Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (AB2739)

  • 5
  • 18
Primary Antibodies

AB18256

Anti-HMGB1 antibody

KO Validated

Western blot - Anti-HMGB1 antibody (AB18256)

  • 5
  • 92

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