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リコンビナントRabMAb

Anti-U2AF35/U2AF1 抗体 [EPR12649(B)] (ab172614)

Reviews (1) Submit a question References (5)
Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-U2AF35/U2AF1 antibody [EPR12649(B)]
    U2AF35/U2AF1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IPmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, 293T, Ramos and NIH 3T3 cell lysates; HeLa cells; Human endometrial carcinoma and Human pancreas tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12649(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab172614の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
特記事項
WB
1/1000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

  • 配列類似性

    Belongs to the splicing factor SR family.
    Contains 2 C3H1-type zinc fingers.
    Contains 1 RRM (RNA recognition motif) domain.

  • ドメイン

    The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus. Nucleus speckle.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DKFZp313J1712 antibody
    • FP793 antibody
    • Human U2 snRNP auxiliary factor small subunit9 antibody
    • RN antibody
    • RNU2AF1 antibody
    • Splicing factor U2AF 35 kDa subunit antibody
    • U2 auxiliary factor 35 kDa subunit antibody
    • U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit antibody
    • U2 small nuclear RNA auxiliary factor 1 antibody
    • U2 snRNP auxiliary factor small subunit antibody
    • U2(RNU2) small nuclear RNA auxiliary factor 1 antibody
    • U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody
    • U2AF1 antibody
    • U2AF1_HUMAN antibody
    • U2AF35 antibody
    • U2AFBP antibody
    see all

画像

  • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
    Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
    All lanes : Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : NIH/3T3 cell lysate
    Lane 4 : Ramos cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
    Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

    Immunofluorescent analysis of HeLa cells labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

  • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
    Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab172614 at 1/10 dilution.

  • Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
    Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

参考文献 (5)

ab172614 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab172614 は 5 報の論文で使用されています。

  • Boudreault S  et al. Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components. Nucleic Acids Res 50:5263-5281 (2022). PubMed: 35489070
  • Leader Y  et al. The upstream 5' splice site remains associated to the transcription machinery during intron synthesis. Nat Commun 12:4545 (2021). PubMed: 34315864
  • Laaref AM  et al. The core spliceosomal factor U2AF1 controls cell-fate determination via the modulation of transcriptional networks. RNA Biol 17:857-871 (2020). PubMed: 32150510
  • Esfahani MS  et al. Functional significance of U2AF1 S34F mutations in lung adenocarcinomas. Nat Commun 10:5712 (2019). PubMed: 31836708
  • Palangat M  et al. The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation. Genes Dev 33:482-497 (2019). PubMed: 30842218

レビューと Q&A

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Western blot abreview for Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (BHK21-T7)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
BHK21-T7
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Simon Boudreault

Verified customer

投稿 Sep 16 2020

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