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リコンビナントRabMAb

Anti-U1-C 抗体 [EPR16034] - BSA and Azide free (ab246328)

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Immunoprecipitation - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
  • Immunocytochemistry/ Immunofluorescence - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
  • Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16034] to U1-C - BSA and Azide free
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-U1-C antibody [EPR16034] - BSA and Azide free
    U1-C 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR16034] to U1-C - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562, Jurkat, HeLa, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates: Human tonsil tissue, Human squamous cell carcinoma of cervix tissue and Rat liver tissue; K562 cells.

  • 特記事項

    ab246328 is the carrier-free version of ab192028.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR16034

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab246328の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

画像

  • Immunoprecipitation - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
    Immunoprecipitation - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)

    Western blot analysis of U1-C in K562 lysate immunoprecipitated using ab192028 at 1/40 dilution (Lane 1).  Lane 2: PBS instead of K562 lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Blocking/dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192028).

  • Immunocytochemistry/ Immunofluorescence - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
    Immunocytochemistry/ Immunofluorescence - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised HeLa cells labeling U1-C with ab192028 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution (green). The nuclear counter stain is Dapi (blue). 

    The two negative controls are ab192028 at 1/500 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192028).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192028).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192028).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192028).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)
    Anti-U1-C antibody [EPR16034] - BSA and Azide free (ab246328)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab246328 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab246328 は論文での使用が確認できていません。

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