AB6211

Anti-Tyrosine Hydroxylase 抗体

Name: Anti-Tyrosine Hydroxylase 抗体 (ab6211) | Abcam
Brand: Abcam Limited
SKU: AB6211
Price: 68000 JPY
Availability: InStock
Rating: 5 (5 reviews)

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

(5 Reviews)

(99 Publications)

Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody detecting Tyrosine Hydroxylase in Western Blot, IHC-Fr, ICC/IF. Suitable for Mouse, Rat.

- Over 70 publications
- Trusted since 2000

別名を表示する

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.

Image courtesy of QBM Cell Sciences, Ottawa, Canada

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1 : 100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab6211) at 1/100 dilution

All lanes:

PC12 cell lysates at 6 µg

Predicted band size: 58 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

ICC/IF, WB, IHC-FoFr, IHC-Fr

applications

免疫原

Synthetic Peptide within Rat TH aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P04177

特異性

Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.

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Reactivity data

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製品の詳細

Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-Fr, WB in mouse samples.

Anti-Tyrosine Hydroxylase antibody (ab6211) has been cited over 77 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Tyrosine Hydroxylase antibody (ab6211) has high sensitivity and specificity.

Anti-Tyrosine Hydroxylase antibody (ab6211) has 4 independent reviews from customers.

Anti-Tyrosine Hydroxylase antibody (ab6211) specifically detects Tyrosine Hydroxylase (UniProt ID: P04177; Molecular weight: 56kDa) and is sold in a 50 µL selling size.

This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Whole antiserum

バッファー組成

pH: 7 - 7.4 Constituents: Whole serum

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed : 30936473).

See full target information Th

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文献 (99)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1453 PubMed41068261

2025

HSPA4 restrains transferrin in dopaminergic neurons to attenuate ferroptosis in a Parkinson's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Gao,Huanhuan Wei,Qianqian Ju,Yongqi Lin,Xiang Yin,Xiaoyu Liu,Jianhong Shen,Qiuhong Ji,Cheng Sun,Lihua Shen

Nature communications 16:7033 PubMed40908286

2025

Identification of Neuritin 1 as a local metabolic regulator of brown adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Sánchez-Feutrie,Montserrat Romero,Sónia R Veiga,Núria Borràs-Ferré,Nick Berrow,Martina Ràfols,Noemí Giménez,Andrea Rodgers-Furones,Alba Sabaté-Pérez,Ángela Rodríguez Pérez,Luis Rodrigo Cataldo,Hans Burghardt,David Sebastián,Natàlia Plana,Vanessa Hernández,Laura Isabel Alcaide,Óscar Reina,Maria J Monte,José Juan G Marin,Manuel Palacín,Remy Burcelin,Per Antonson,Jan-Ake Gustafsson,Antonio Zorzano

Molecules (Basel, Switzerland) 30: PubMed40430379

2025

Protective Role of Whey Protein Isolate on MPP-Induced Differentiation of SH-SY5Y Cells by Modulating the Nrf2 Antioxidant Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Panlekha Rungruang,Morakot Sroyraya,Veerawat Sansri

Frontiers in aging neuroscience 17:1503168 PubMed40201545

2025

Whole-brain 3D imaging of dopaminergic neurons and glial cells in the mouse model of Parkinson's disease induced by 6-OHDA.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Wang,Linglong Xiao,Yifeng Shi,Yaping Wu,Xinyuejia Huang,Yang Wu,Yangyang Xu,Lin Bai,Wei Pan,Jie Zhang,Wei Wang

Acta neuropathologica communications 13:59 PubMed40083022

2025

Cranial radiation disrupts dopaminergic signaling and connectivity in the mammalian brain.

Applications

Unspecified application

Species

Unspecified reactive species

Die Zhang,Riya Thomas,Thanh Thai Lam,Ines Veselinovic,David R Grosshans

Vavilovskii zhurnal genetiki i selektsii 28:744-751 PubMed39722665

2024

Substantia nigra alterations in mice modeling Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

I N Rozhkova,S V Okotrub,E Yu Brusentsev,T A Rakhmanova,D A Lebedeva,V S Kozeneva,N A Shavshaeva,N V Khotskin,S Ya Amstislavsky

eLife 13: PubMed39671236

2024

Projection neurons are necessary for the maintenance of the mouse olfactory circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Luis Sánchez-Guardado,Peyman Razavi,Bo Wang,Antuca Callejas-Marín,Carlos Lois

Animal bioscience 38:236-246 PubMed39210791

2024

Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Chen,Tingting Lu,Yingying Dai,Yu Xue,Bohao Zhao,Xinsheng Wu

ACS nano 18:13277-13285 PubMed38728175

2024

Multifunctional and Flexible Neural Probe with Thermally Drawn Fibers for Bidirectional Synaptic Probing in the Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yeji Kim,Yunheum Lee,Jeongeun Yoo,Kum Seok Nam,Woojin Jeon,Seungmin Lee,Seongjun Park

Neuron 112:1978-1996.e6 PubMed38599212

2024

Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Saptarshi Biswas,Sanjid Shahriar,Galina Bachay,Panos Arvanitis,Danny Jamoul,William J Brunken,Dritan Agalliu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tyrosine Hydroxylase 抗体

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (99)

Abcam product promise