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AB314080

Anti-Tyrosine Hydroxylase 抗体 [BLR155J] - BSAfree

Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree

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Rabbit Recombinant Monoclonal Tyrosine Hydroxylase antibody. Suitable for ICC, IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TH aa 450 to C-terminus.

別名を表示する

TYH, TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase

5 Images
Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)

Detection of human Tyrosine Hydroxylase in FFPE SK-NBE(2) cells by immunocytochemistry. Antibody : Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)

Detection of human Tyrosine Hydroxylase in FFPE mouse brain by immunohistochemistry. Antibody : Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)

Detection of human Tyrosine Hydroxylase in FFPE mouse olfactory bulb by immunohistochemistry. Antibody : Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)
  • WB

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)

Samples : Whole cell lysate (10 µg) from HEK293T, SKN-SH, SK-N-BE(2), Jurkat, and SK-N-MC cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 10 µg

Lane 2:

SKN-SH Whole cell lysate at 10 µg

Lane 3:

SK-N-BE(2) Whole cell lysate at 10 µg

Lane 4:

Jurkat Whole cell lysate at 10 µg

Lane 5:

SK-N-MC Whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)
  • WB

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080)

Samples : Whole cell lysate (50 µg) from NIH 3T3, CT26, ND7/23, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

CT26 Whole cell lysate at 50 µg

Lane 3:

ND7/23 Whole cell lysate at 50 µg

Lane 4:

TCMK-1 Whole cell lysate at 50 µg

Lane 5:

BW5147.3 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BLR155J

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

IHC-P, ICC, WB

applications

免疫原

Synthetic Peptide within Human TH aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07101

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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出荷温度及び保存条件

製品の状態
Liquid
バッファー組成
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
出荷温度
Blue Ice
短期保存期間
Up to 12 months
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed : 15287903, PubMed : 1680128, PubMed : 17391063, PubMed : 24753243, PubMed : 34922205, PubMed : 8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).. Isoform 5. Lacks catalytic activity.. Isoform 6. Lacks catalytic activity.
See full target information TH
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組合せ製品

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-c-Fos antibody [EPR883(2)]

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Abcam product promise

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