Anti-Tyrosine Hydroxylase 抗体 (ab76442)
Key features and details
- Chicken polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-P, IHC-FrFl
- Reacts with: Mouse
- Isotype: IgY
製品の概要
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製品名
Anti-Tyrosine Hydroxylase antibody
Tyrosine Hydroxylase 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to Tyrosine Hydroxylase -
由来種
Chicken -
アプリケーション
適用あり: IHC-P, IHC-FrFlmore details
適用なし: WB -
種交差性
交差種: Mouse -
免疫原
Two synthetic peptide/ keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101) and mouse (P24529) sequences.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.02% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Two different affinity-purified anti-peptide antibodies were combined to make this product. After repeated injections of synthetic peptides into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 100 ug/ml. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized. The concentrations of both antibodies were 100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard), making the total antibody concentration 200 ug/ml. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76442の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (3) |
1/1000 - 1/2000.
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| IHC-FrFl | (3) |
1/1000.
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| 特記事項 |
|---|
|
IHC-P
1/1000 - 1/2000. |
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IHC-FrFl
1/1000. |
ターゲット情報
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機能
Plays an important role in the physiology of adrenergic neurons. -
組織特異性
Mainly expressed in the brain and adrenal glands. -
パスウェイ
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
関連疾患
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
配列類似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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参照データベース
- Entrez Gene: 21823 Mouse
- SwissProt: P24529 Mouse
- Unigene: 1292 Mouse
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別名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
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Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (160)
ab76442 は 160 報の論文で使用されています。
- Tseng CT et al. Frequency Specific Optogenetic Stimulation of the Locus Coeruleus Induces Task-Relevant Plasticity in the Motor Cortex. J Neurosci 44:N/A (2024). PubMed: 38124020
- Lin G et al. Exploring therapeutic strategies for infantile neuronal axonal dystrophy (INAD/PARK14). Elife 12:N/A (2023). PubMed: 36645408
- Seo DC et al. DDC-Promoter-Driven Chemogenetic Activation of SNpc Dopaminergic Neurons Alleviates Parkinsonian Motor Symptoms. Int J Mol Sci 24:N/A (2023). PubMed: 36768816
- Parrales-Macias V et al. The Pesticide Chlordecone Promotes Parkinsonism-like Neurodegeneration with Tau Lesions in Midbrain Cultures and C. elegans Worms. Cells 12:N/A (2023). PubMed: 37174736
- Rájová J et al. Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo. Mol Ther Methods Clin Dev 29:381-394 (2023). PubMed: 37251982