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リコンビナントRabMAb

Anti-Tyrosinase 抗体 [EPR10141] (ab170905)

Reviews (1) Submit a question References (20)
Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (ab170905)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (ab170905)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [EPR10141] (ab170905)
  • Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
  • Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
  • Flow Cytometry (Intracellular) - Anti-Tyrosinase antibody [EPR10141] (ab170905)
  • Anti-Tyrosinase antibody [EPR10141] (ab170905)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10141] to Tyrosinase
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-Tyrosinase antibody [EPR10141]
    Tyrosinase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10141] to Tyrosinase

  • 由来種

    Rabbit

  • 特異性

    This antibody does not react with mouse species in Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) application.

  • アプリケーション

    適用あり: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment within Human Tyrosinase. The exact sequence is proprietary.
    Database link: P14679

  • ポジティブ･コントロール

    • WB: Human melanoma lysate, SK-MEL-28. MeWo, B16-F0 and B16-F10 whole cell lysates. IHC-P: Human melanoma and skin tissue. ICC/IF: A375 cells. Flow Cyt (Intra): A375 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab170905の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/30.
特記事項
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
ICC/IF
1/50 - 1/100.
Flow Cyt (Intra)
1/30.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.

  • 関連疾患

    Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.

  • 配列類似性

    Belongs to the tyrosinase family.

  • 細胞内局在

    Melanosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ATN antibody
      • CMM8 antibody
      • LB24 AB antibody
      • LB24-AB antibody
      • Monophenol monooxygenase antibody
      • OCA1 antibody
      • OCA1A antibody
      • OCAIA antibody
      • Oculocutaneous albinism IA antibody
      • SHEP3 antibody
      • SK29 AB antibody
      • SK29-AB antibody
      • Tumor rejection antigen AB antibody
      • TYR antibody
      • TYRO_HUMAN antibody
      • tyrosinase (oculocutaneous albinism IA) antibody
      • Tyrosinase antibody
      see all

    画像

    • Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      All lanes : Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution

      Lane 1 : B16-F0 (mouse melanoma epithelial-like cell), whole cell lysate
      Lane 2 : B16-F10 (mouse melanoma epithelial-like cell), whole cell lysate
      Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 4 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 60 kDa
      Observed band size: 50-80 kDa why is the actual band size different from the predicted?


      Exposure time: 59 seconds


      Blocking buffer and concentration: 5% NFDM/TBST
      Diluting buffer and concentration: 5% NFDM/TBST

      The expression profile observed is consistent with what has been described in the literature (PMID: 20053998; PMID: 24933607).
      Negative control: NIH/3T3, RAW264.7 whole cell lysates.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (ab170905)

      Immunohistochemical analysis of paraffin-embedded human skin tissue labelling Tyrosinase with ab170905 at 1/500 (0.662 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skin. The section was incubated with ab170905 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (ab170905)

      Immunohistochemical analysis of paraffin-embedded Human melanoma tissue, labeling Tyrosinase using ab170905 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody [EPR10141] (ab170905)

      ab170905 staining Tyrosinase in A375 (human malignant melanoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Control: PBS only.

    • Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      All lanes : Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution

      Lane 1 : SK-MEL-28 (Human malignant melanoma) whole cell lysate
      Lane 2 : MeWo (Human malignant melanoma fibroblast) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST.

      Exposure: 

      Lane 1: 20 seconds

      Lane 2: 60 seconds

    • Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Western blot - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution + Human melanoma lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 60 kDa

    • Flow Cytometry (Intracellular) - Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Flow Cytometry (Intracellular) - Anti-Tyrosinase antibody [EPR10141] (ab170905)

      Intracellular Flow Cytometry analysis of A375 (human malignant melanoma) cells labeling with purified ab170905 at 1/30 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control. 

       

       

       

       

    • Anti-Tyrosinase antibody [EPR10141] (ab170905)
      Anti-Tyrosinase antibody [EPR10141] (ab170905)

    参考文献 (20)

    ab170905 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab170905 は 20 報の論文で使用されています。

    • Ferreira Branquinho MS  et al. Kynurenine inhibits melanogenesis in human melanocyte-keratinocyte co-cultures and in a reconstructed 3D skin model. Exp Dermatol 31:427-432 (2022). PubMed: 34710259
    • Hu SH  et al. sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes. Oxid Med Cell Longev 2022:1856830 (2022). PubMed: 35186181
    • Wu X  et al. Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo. Pigment Cell Melanoma Res 35:328-341 (2022). PubMed: 35218147
    • Liu Y  et al. Ablation of Proton/Glucose Exporter SLC45A2 Enhances Melanosomal Glycolysis to Inhibit Melanin Biosynthesis and Promote Melanoma Metastasis. J Invest Dermatol 142:2744-2755.e9 (2022). PubMed: 35469906
    • Kamo H  et al. Tyrosinase suppresses vasculogenic mimicry in human melanoma cells. Oncol Lett 23:169 (2022). PubMed: 35496574
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-Tyrosinase antibody [EPR10141]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse melanoma (B16F10), human melanoma (SKMel-3),)
    Gel Running Conditions
    Reduced Denaturing (Criterion TGX 4- 15% (BioRad)))
    Loading amount
    40 µg
    Specification
    mouse melanoma (B16F10), human melanoma (SKMel-3),
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mercedes Quintana

    Verified customer

    投稿 Sep 30 2016

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