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リコンビナントRabMAb

Anti-TXNRD1 抗体 [EPR10205] (ab167411)

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Western blot - Anti-TXNRD1 antibody [EPR10205] (ab167411)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
  • Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPR10205] (ab167411)
  • Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
  • Anti-TXNRD1 antibody [EPR10205] (ab167411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10205] to TXNRD1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TXNRD1 antibody [EPR10205]
    TXNRD1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10205] to TXNRD1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
    適用なし: IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, SH-SY5Y and HeLa cell lysates; Human pancreas tissue; HepG2 cells; permeabilized SH-SY5Y cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab167411の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 71 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 71 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.

  • 組織特異性

    Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.

  • 配列類似性

    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.

  • ドメイン

    The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.

  • 翻訳後修飾

    The N-terminus of isoform 5 is blocked.
    ISGylated.

  • 細胞内局在

    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • cytoplasmic antibody
      • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
      • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
      • Gene associated with retinoid IFN induced mortality 12 protein antibody
      • GRIM 12 antibody
      • GRIM-12 antibody
      • GRIM12 antibody
      • KDRF antibody
      • KM 102 derived reductase like factor antibody
      • KM-102-derived reductase-like factor antibody
      • MGC9145 antibody
      • Oxidoreductase antibody
      • Thioredoxin reductase 1 antibody
      • Thioredoxin reductase 1 cytoplasmic antibody
      • Thioredoxin reductase GRIM 12 antibody
      • Thioredoxin reductase TR1 antibody
      • TR 1 antibody
      • TR antibody
      • TR1 antibody
      • TRXR 1 antibody
      • TRXR1 antibody
      • TRXR1_HUMAN antibody
      • TXNR antibody
      • TXNRD 1 antibody
      • Txnrd1 antibody
      see all

    画像

    • Western blot - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Western blot - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      All lanes : Anti-TXNRD1 antibody [EPR10205] (ab167411) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 71 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)

      ab167411 showing +ve staining in Human normal kidney.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)

      ab167411 showing +ve staining in Human heart.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)

      ab167411 showing +ve staining in Human normal liver.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] (ab167411)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TXNRD1 with ab167411 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Immunofluorescent staining of HepG2 cells labeling TXNRD1 with ab167411 at 1/250 dilution. DAPI nuclear staining (blue).
    • Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPR10205] (ab167411)

      Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling TXNRD1 with ab167411 at 1/100 dilution (red) or with a rabbit IgG (negative) (green).

       

    • Anti-TXNRD1 antibody [EPR10205] (ab167411)
      Anti-TXNRD1 antibody [EPR10205] (ab167411)

    参考文献 (0)

    ab167411 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab167411 は論文での使用が確認できていません。

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