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リコンビナントRabMAb

Anti-TXNRD1 抗体 [EPNCIR129] - BSA and Azide free (ab187363)

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Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
  • Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
  • Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
  • Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
  • Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR129] to TXNRD1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 HRP Unconjugated

製品の概要

  • 製品名

    Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free
    TXNRD1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPNCIR129] to TXNRD1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt (Intra), IP, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant full length protein corresponding to Mouse TXNRD1.
    Database link: Q9JMH6

  • 特記事項

    ab187363 is the carrier-free version of ab124954.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187363の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55, 67 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55, 67 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.

  • 組織特異性

    Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.

  • 配列類似性

    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.

  • ドメイン

    The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.

  • 翻訳後修飾

    The N-terminus of isoform 5 is blocked.
    ISGylated.

  • 細胞内局在

    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • cytoplasmic antibody
    • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
    • Gene associated with retinoid IFN induced mortality 12 protein antibody
    • GRIM 12 antibody
    • GRIM-12 antibody
    • GRIM12 antibody
    • KDRF antibody
    • KM 102 derived reductase like factor antibody
    • KM-102-derived reductase-like factor antibody
    • MGC9145 antibody
    • Oxidoreductase antibody
    • Thioredoxin reductase 1 antibody
    • Thioredoxin reductase 1 cytoplasmic antibody
    • Thioredoxin reductase GRIM 12 antibody
    • Thioredoxin reductase TR1 antibody
    • TR 1 antibody
    • TR antibody
    • TR1 antibody
    • TRXR 1 antibody
    • TRXR1 antibody
    • TRXR1_HUMAN antibody
    • TXNR antibody
    • TXNRD 1 antibody
    • Txnrd1 antibody
    see all

画像

  • Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
    Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)

    ab124954 (purified) at 1/40 immunoprecipitating TXNRD1 in 10 µg Jurkat (Lanes 1 and 2, observed at 55 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

  • Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
    Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)

    Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab124954 at a dilution of 1 in 120 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
    Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)

    Immunofluorescence staining of HeLa cells with purified ab124954 at a working dilution of 1/150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124954 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
    Immunohistochemical staining of paraffin embedded human testis with purified ab124954 at a working dilution of 1/150. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

  • Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
    Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)

    Unpurified ab124954, at 1/100 dilution, staining TXNRD1 in HeLa cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

  • Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)
    Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (ab187363)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab187363 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187363 は論文での使用が確認できていません。

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