AB187697

Anti-TTLL5 抗体

Name: Anti-TTLL5 抗体 (ab187697) | Abcam
Brand: Abcam Limited
SKU: AB187697
Price: 70000 JPY
Availability: InStock

Anti-TTLL5 antibody

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(2 Publications)

Rabbit Polyclonal TTLL5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TTLL5 aa 1200 to C-terminus.

別名を表示する

KIAA0998, STAMP, TTLL5, Tubulin polyglutamylase TTLL5, SRC1 and TIF2-associated modulatory protein, Tubulin--tyrosine ligase-like protein 5, STAMP protein

2 Images

Supplier Data

Immunoprecipitation - Anti-TTLL5 antibody (AB187697)

Detection of TTLL5 by Western Blot of Immunprecipitate.

Lane 1 : ab187697 1μg/ml labeling TTLL5 in HeLa whole cell lysate immunoprecipitated using ab187697 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-TTLL5 antibody (ab187697)

Predicted band size: 144 kDa

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Supplier Data

Western blot - Anti-TTLL5 antibody (AB187697)

All lanes:

Western blot - Anti-TTLL5 antibody (ab187697) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 144 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human TTLL5 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6EMB2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TTLL5 or Tubulin Tyrosine Ligase Like 5 acts as an enzyme that adds glutamates onto tubulin a process known as polyglutamylation. It has an approximate mass of 121 kDa. This protein is expressed in various tissues with higher levels observed in the retinal photoreceptor cells and testes. TTLL5 plays a role in the post-translational modification of proteins which is essential for the functioning of the microtubule network.

Biological function summary

TTLL5 contributes to cellular processes by modifying tubulin a critical component of the cytoskeleton. In its role it performs polyglutamylation affecting microtubule stability and interactions with microtubule-associated proteins (MAPs) and motor proteins. TTLL5 functions as a part of a larger group of enzymes similar to other members of the tubulin tyrosine ligase-like family which regulates the different aspects of microtubule dynamics.

Pathways

TTLL5 is important in the MAPK signaling pathway and axon guidance. In these pathways TTLL5 affects cellular morphology and signaling by modifying tubulin structures which may regulate the activity of proteins like MAP2 and tau impacting cell transport and division. Its role in these pathways highlights the significance of microtubule modifications in both cellular signaling and structural integrity.

Mutations or dysregulation of TTLL5 have been linked to retinal degeneration and ciliary dysfunction disorders. In retinal degeneration TTLL5 defects affect photoreceptor cell functions due to improper protein modification. The protein’s impact on the disease connects through interaction with other microtubule-related proteins which are also critical for maintaining retinal cell structure and function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Polyglutamylase which modifies tubulin, generating polyglutamate side chains on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Preferentially mediates ATP-dependent initiation step of the polyglutamylation reaction over the elongation step. Preferentially modifies the alpha-tubulin tail over a beta-tail (By similarity). Required for CCSAP localization to both polyglutamylated spindle and cilia microtubules (PubMed : 22493317). Increases the effects of transcriptional coactivator NCOA2/TIF2 in glucocorticoid receptor-mediated repression and induction and in androgen receptor-mediated induction (PubMed : 17116691).

See full target information TTLL5

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70245 PubMed39648156

2024

KLF7 Promotes Hepatocellular Carcinoma Progression Through Regulating SLC1A5-Mediated Tryptophan Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Chai,Anhong Zhang,Yang Liu,Xi Zhang,Pengzhou Kong,Zhuowei Zhang,Yarong Guo

Cell discovery 8:77 PubMed35945223

2022

GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Shupei Wei,Qin Zhao,Ke Zheng,Peiying Liu,Nannan Sha,Yingzi Li,Chunmin Ma,Jingjie Li,Lingang Zhuo,Guanxin Liu,Wenhua Liang,Yuhui Jiang,Tao Chen,Nanshan Zhong

View all publications

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TTLL5 抗体

Anti-TTLL5 antibody

Immunoprecipitation - Anti-TTLL5 antibody (AB187697)

Western blot - Anti-TTLL5 antibody (AB187697)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Abcam product promise