Anti-TTK/Mps1 抗体 [N1] - BSA free (ab11108)
Key features and details
- Mouse monoclonal [N1] to TTK/Mps1 - BSA free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-TTK/Mps1 antibody [N1] - BSA free
TTK/Mps1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N1] to TTK/Mps1 - BSA free -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human TTK/Mps1 aa 1-900. The exact sequence is proprietary. (catalytically inactive - D663A)
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エピトープ
This antibody recognizes an epitope in the N-terminal region of Mps1. -
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N1 -
ミエローマ
PAIB3Ag81 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11108の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). NOT SUITABLE for blocking with BSA. Block in 5% milk for 1 hour.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa). NOT SUITABLE for blocking with BSA. Block in 5% milk for 1 hour. |
ターゲット情報
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機能
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint. -
組織特異性
Present in rapidly proliferating cell lines. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 7272 Human
- Omim: 604092 Human
- SwissProt: P33981 Human
- Unigene: 169840 Human
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別名
- cancer/testis antigen 96 antibody
- CT96 antibody
- Dual specificity protein kinase TTK antibody
see all
画像
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All lanes : Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
NOTE: MILK was used to block non-specific binding, rather than BSA. -
Anti-TTK/Mps1 antibody [N1] - BSA free (ab11108) at 10 µg/ml +
Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 95 kDa
Exposure time: 8 minutesab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab11108 は 27 報の論文で使用されています。
- Jinteng L et al. BMAL1-TTK-H2Bub1 loop deficiency contributes to impaired BM-MSC-mediated bone formation in senile osteoporosis. Mol Ther Nucleic Acids 31:568-585 (2023). PubMed: 36910712
- Sarwar S et al. Inhibition of Mps1 kinase enhances taxanes efficacy in castration resistant prostate cancer. Cell Death Dis 13:868 (2022). PubMed: 36229449
- Kitajima S et al. MPS1 inhibition primes immunogenicity of KRAS-LKB1 mutant lung cancer. Cancer Cell 40:1128-1144.e8 (2022). PubMed: 36150391
- Fang Q et al. The essential roles of Mps1 in spermatogenesis and fertility in mice. Cell Death Dis 12:531 (2021). PubMed: 34031364
- Xia P et al. Reversine inhibits proliferation, invasion and migration and induces cell apoptosis in gastric cancer cells by downregulating TTK. Exp Ther Med 22:929 (2021). PubMed: 34306198