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AB24226

Anti-TTK/Mps1 抗体

Anti-TTK/Mps1 antibody

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(1 Publication)

Rabbit Polyclonal TTK/Mps1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TTK aa 700-750.

別名を表示する

MPS1, MPS1L1, TTK, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase, PYT

1 Images
Western blot - Anti-TTK/Mps1 antibody (AB24226)
  • WB

Unknown

Western blot - Anti-TTK/Mps1 antibody (AB24226)

Detection of Human TTK/Mps1 by Western Blot and Immunoprecipitation.

Samples : Whole cell lysate (15 and 50 μg for A; 50 μg for B; 2 mg for B) from HeLa cells. In B, HeLa cells were treated with an siRNA for vimentin (control) or TTK/Mps1 ab24226 used at 0.033 (A) and 0.33 (B) μg/ml for WB and at 1 μg/2 mg lysate for IP (C). TTK/Mps1 was also immunoprecipitated with ab24226 using 1 μg/2 mg lysate. IP reactions were controlled using normal rabbit IgG (control IP). Immunoprecipitated TTK/Mps1 was blotted using a goat antibody directed to an upstream epitope on TTK/Mps1. Detection : Chemiluminescence with exposure times of 1 minute (A), 15 seconds (B) and 30 seconds (C).

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Western blot - Anti-TTK/Mps1 antibody (ab24226)

Predicted band size: 97 kDa

Observed band size: 95 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human TTK aa 700-750. The exact immunogen used to generate this antibody is proprietary information.

P33981

特異性

ab24226 reacts with TTK (Msp1) protein.

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Reactivity data

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Antibody was affinity purified using an epitope specific to TTK immobilized on solid support.
バッファー組成
Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TTK also known as Mps1 is a dual-specificity protein kinase with a molecular mass of approximately 97 kDa. It plays a mechanical role in the regulation of the cell cycle by phosphorylating various substrates required for accurate chromosome alignment and segregation during mitosis. TTK/Mps1 is expressed in many tissues but shows the highest levels in rapidly dividing cells such as in the testis and in tumors. The presence of this protein in proliferative tissues suggests its involvement in cell cycle control mechanisms.
Biological function summary

TTK/Mps1 protein is essential for maintaining genomic stability. It functions as part of the mitotic checkpoint complex (MCC) which prevents cells from prematurely advancing from metaphase to anaphase until all chromosomes are correctly aligned on the mitotic spindle. By doing so TTK/Mps1 ensures proper chromosomal attachment and tension at kinetochores reducing the likelihood of aneuploidy. This regulation highlights its critical role in preventing genetic variation during cell division.

Pathways

TTK/Mps1 is an important component of the spindle assembly checkpoint (SAC) pathway which plays an important role in cell cycle regulation. It works closely with other proteins such as BUBR1 BUB3 and MAD2 to halt cell cycle progression until chromosomal alignment is verified. In addition TTK/Mps1 interacts with the aurora kinase A (AURKA) pathway which is instrumental in centrosome maturation and spindle assembly further emphasizing its role in ensuring accurate mitosis.

Aberrations in TTK/Mps1 activity are associated with cancer and aneuploidy. Overexpression or amplification of TTK/Mps1 is frequently observed in various tumors linking it with cancer progression and poor prognosis. Its interaction with other proteins like p53 a well-known tumor suppressor is significant in understanding its role in oncogenesis. Therapeutic targeting of TTK/Mps1 is being explored to exploit its critical function in rapidly proliferating cancer cells aiming to induce mitotic catastrophe and mitigate tumor growth.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed : 18243099, PubMed : 28441529, PubMed : 29162720). Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint (PubMed : 18243099, PubMed : 29162720). Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore (PubMed : 18243099). Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments (PubMed : 28441529). Phosphorylates KNL1, KNTC1 and autophosphorylates (PubMed : 28441529). Phosphorylates MCRS1 which enhances recruitment of KIF2A to the minus end of spindle microtubules and promotes chromosome alignment (PubMed : 30785839).
See full target information TTK
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 11: PubMed37631871

2023

Maternal and Infant Histo-Blood Group Antigen (HBGA) Profiles and Their Influence on Oral Rotavirus Vaccine (Rotarix) Immunogenicity among Infants in Zambia.

Applications

Unspecified application

Species

Unspecified reactive species

Adriace Chauwa,Samuel Bosomprah,Natasha Makabilo Laban,Bernard Phiri,Mwelwa Chibuye,Obvious Nchimunya Chilyabanyama,Sody Munsaka,Michelo Simuyandi,Innocent Mwape,Cynthia Mubanga,Masuzyo Chirwa Chobe,Caroline Chisenga,Roma Chilengi
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代替製品

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