Anti-TTC5 抗体 (ab36855)
Key features and details
- Rabbit polyclonal to TTC5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TTC5 antibody -
製品の詳細
Rabbit polyclonal to TTC5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
A 14 amino acid peptide from near the carboxy terminus of human TTC5.
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ポジティブ･コントロール
- RAW264.7 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36855の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Tetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats. Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5, suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs. -
細胞内局在
Nucleus. Cytoplasm. Note: Phosphorylation at Ser-203 results in nuclear localization, while unphosphorylated protein localizes to the cytoplasm -
参照データベース
- Entrez Gene: 91875 Human
- Entrez Gene: 219022 Mouse
- SwissProt: Q8N0Z6 Human
- SwissProt: Q99LG4 Mouse
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別名
- Strap antibody
- Stress-responsive activator of p300 antibody
- Tetratricopeptide repeat domain 5 antibody
- TPR repeat protein 5 antibody
画像
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Western blot - Anti-TTC5 antibody (ab36855)Lane 1 : Anti-TTC5 antibody (ab36855) at 0.5 µg/ml
Lane 2 : Anti-TTC5 antibody (ab36855) at 1 µg/ml
Lane 3 : Anti-TTC5 antibody (ab36855) at 2 µg/ml
Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lanes 2-3 : RAW264.7 cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC5 antibody (ab36855)ab36855 at 10µg/ml staining TCC5 in mouse brain tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-TTC5 antibody (ab36855)ICC/IF image of ab36855 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor®594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-TTC5 antibody (ab36855)Immunofluorescence of TTC5 in Mouse Brain cells using ab36855 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab36855 は 1 報の論文で使用されています。
- Davies L et al. Regulation of glucocorticoid receptor activity by a stress responsive transcriptional cofactor. Mol Endocrinol 25:58-71 (2011). WB ; Human . PubMed: 21147850