AB197076

Anti-TTC28/TPRBK 抗体 - C-terminal

Name: Anti-TTC28/TPRBK 抗体 - C-terminal (ab197076) | Abcam
Brand: Abcam Limited
SKU: AB197076
Price: 73000 JPY
Availability: InStock

Anti-TTC28/TPRBK antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal TTC28/TPRBK antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TTC28 aa 2450 to C-terminus.

別名を表示する

KIAA1043, TPRBK, TTC28, Tetratricopeptide repeat protein 28, TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio

2 Images

Supplier Data

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Detection of TTC28/TPRBK in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197076 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (ab197076)

Predicted band size: 271 kDa

false

Supplier Data

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Lysates prepared wtih NETN lysis buffer.

All lanes:

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (ab197076) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 271 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human TTC28 aa 2450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96AY4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab197076 was affinity purified using an epitope specific to TTC28/TPRBK immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The TTC28 also known as TPRBK is a protein with a molecular weight of approximately 230 kDa. This protein consists of tetratricopeptide repeat domains that are important for protein-protein interactions. TTC28 exhibits a broad expression pattern in human tissues but shows higher expression levels in the brain testis and certain cancerous tissues. Its widespread expression suggests a fundamental role across various biological systems.

Biological function summary

TTC28/TPRBK may influence cellular processes including cell cycle regulation and protein folding. This protein interacts with other proteins as part of a multiprotein complex including components of the cytoskeleton and cell cycle regulators. Its participation in these important processes suggests that TTC28 plays a role in maintaining cellular integrity and function.

Pathways

When considering the cellular mechanism TTC28/TPRBK participates in the cell cycle regulation pathway and possibly impacts cell division. It shows interaction with proteins like cyclins and cyclin-dependent kinases which are central to the progression of the cell cycle. These associations indicate that TTC28's function in pathways reflects its functional importance in regulating proper cellular division and growth.

Researchers associate TTC28/TPRBK with specific types of cancer such as glioblastoma and breast cancer. Aberrant expression or dysfunction of TTC28 may disrupt normal cell cycle progression leading to uncontrolled cell proliferation. Its interaction with oncoproteins such as Cyclin D1 further corroborates its potential role in oncogenesis. Understanding TTC28's involvement in these diseases can contribute to developing targeted therapeutic strategies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

During mitosis, may be involved in the condensation of spindle midzone microtubules, leading to the formation of midbody.

See full target information TTC28

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2409447121 PubMed39630868

2024

The essential role of TTC28 in maintaining chromosomal stability via HSPA8 chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Zhang,Meiyi Xiang,Liankun Gu,Jing Zhou,Baozhen Zhang,Wei Tian,Dajun Deng

Neuropsychopharmacology : official publication of 45:1884-1895 PubMed32610340

2020

Activation of tyrosine phosphatase PTP1B in pyramidal neurons impairs endocannabinoid signaling by tyrosine receptor kinase trkB and causes schizophrenia-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohong Qin,Li Zhang,Shelly A Cruz,Alexandre F R Stewart,Hsiao-Huei Chen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-TTC28/TPRBK 抗体 - C-terminal

Anti-TTC28/TPRBK antibody - C-terminal

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise