Anti-TTC19 抗体 [EPR13184-63] (ab181191)
![Western blot - Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.co.jp/ps/products/181/ab181191/Images/ab181191-214659-WB1.png "Western blot - Anti-TTC19 antibody [EPR13184-63] (ab181191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13184-63] to TTC19
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-TTC19 antibody [EPR13184-63]
TTC19 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13184-63] to TTC19 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Human ovarian cancer and HT-1080 cell lysates, HT-1080 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13184-63 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181191の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa).
|
|
| IP |
1/20 - 1/40.
|
|
| ICC/IF |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 42 kDa). |
|
IP
1/20 - 1/40. |
|
ICC/IF
1/50 - 1/100. |
ターゲット情報
-
機能
Mitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence. -
関連疾患
Mitochondrial complex III deficiency, nuclear 2 -
配列類似性
Belongs to the TTC19 family.
Contains 5 TPR repeats. -
細胞内局在
Mitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others. - Information by UniProt
-
参照データベース
- Entrez Gene: 54902 Human
- Omim: 613814 Human
- SwissProt: Q6DKK2 Human
- Unigene: 462316 Human
-
別名
- 2010204O13Rik antibody
- FLJ20343 antibody
- MGC138312 antibody
see all
画像
-
Western blot - Anti-TTC19 antibody [EPR13184-63] (ab181191)All lanes : Anti-TTC19 antibody [EPR13184-63] (ab181191) at 1/5000 dilution
Lane 1 : Human ovarian cancer lysate
Lane 2 : HT-1080 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 42 kDa
Additional bands at: 35 kDa (possible mature (processed) protein) -
![Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.co.jp/ps/products/181/ab181191/Images/ab181191-214658-icc1.png)
Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184-63] (ab181191)Immunocytochemical analysis of HT-1080 cells fixed in 4% paraformaldehyde labeling TTC19 with ab181191 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counterstained with DAP1 (blue)
-
![Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.co.jp/ps/products/181/ab181191/Images/ab181191-214657-ip-copy.png)
Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] (ab181191)Immunoprecipitation of HT-1080 cells labeling TTC19 with ab181191 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
-
![Anti-TTC19 antibody [EPR13184-63] (ab181191)](https://www.abcam.co.jp/ps/products/181/ab181191/Images/ab181191-4-benefits-of-recombinant-antibodies.png)
Anti-TTC19 antibody [EPR13184-63] (ab181191)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab181191 は論文での使用が確認できていません。