Anti-TSLP 抗体 (ab47943)
Key features and details
- Rabbit polyclonal to TSLP
- Suitable for: IHC-P, ICC/IF, WB, ELISA, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TSLP antibody
TSLP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TSLP -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WB, ELISA, Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length TSLP protein (Human),>98% pure
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 100ul of sterile water to 1mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified by affinity chromatography employing immobilized hTSLP matrix. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47943の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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| ELISA |
Use at an assay dependent concentration.
This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution. |
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| Sandwich ELISA |
Use at an assay dependent concentration.
To detect hTSLP by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab84247 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. |
| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
ELISA
Use at an assay dependent concentration. This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution. |
|
Sandwich ELISA
Use at an assay dependent concentration. To detect hTSLP by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab84247 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTSLP. |
ターゲット情報
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機能
Cytokine that induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. -
組織特異性
Expressed in a number of tissues including heart, liver and prostate. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 85480 Human
- Omim: 607003 Human
- SwissProt: Q969D9 Human
- Unigene: 389874 Human
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別名
- Thymic stromal lymphopoietin antibody
- Thymic stromal lymphopoietin protein TSLP antibody
- Tslp antibody
see all
画像
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Sandwich ELISA - Anti-TSLP antibody (ab47943)
ab47943 detecting TSLP in sandwich ELISA
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Western blot - Anti-TSLP antibody (ab47943)Anti-TSLP antibody (ab47943) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 51 kDa, 53 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to TSLP.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab47943 は 30 報の論文で使用されています。
- Numazaki M et al. ASP7266, a Novel Antibody against Human Thymic Stromal Lymphopoietin Receptor for the Treatment of Allergic Diseases. J Pharmacol Exp Ther 380:26-33 (2022). PubMed: 34728559
- Jung SY et al. Wnt-activating human skin organoid model of atopic dermatitis induced by Staphylococcus aureus and its protective effects by Cutibacterium acnes. iScience 25:105150 (2022). PubMed: 36193049
- Hu YQ & Zhang JZ A Comparison for Type 2 Cytokines and Lesional Inflammatory Infiltrations in Bullous Pemphigoid and Atopic Dermatitis. Clin Cosmet Investig Dermatol 15:2313-2321 (2022). PubMed: 36325102
- Jia J et al. An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation. Int J Mol Sci 23:N/A (2022). PubMed: 36362328
- Lenti MV et al. Mucosal Overexpression of Thymic Stromal Lymphopoietin and Proinflammatory Cytokines in Patients With Autoimmune Atrophic Gastritis. Clin Transl Gastroenterol 13:e00510 (2022). PubMed: 35905420