Anti-TSG101 抗体

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody (AB228013)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue stained for TSG101 protein at cytoplasm with ab228013 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody (AB228013)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue stained for TSG101 protein at cytoplasm with ab228013 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IP

Supplier Data

Immunoprecipitation - Anti-TSG101 antibody (AB228013)

TSG101 was immunoprecipitated from A549 (human lung carcinoma cell line from) whole cell extract with 5 μg of ab228013. Western blot was performed from the immunoprecipitate using ab228013. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Control IgG instaed of ab228013 in A549 whole cell extract.

Lane 2 : ab228013 IP in A549 whole cell extract.

All lanes:

Immunoprecipitation - Anti-TSG101 antibody (ab228013)

Predicted band size: 44 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody (AB228013)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-TSG101 antibody (ab228013) at 1/1000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts at 30 µg

Lane 3:

HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody (AB228013)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-TSG101 antibody (ab228013) at 1/500 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody (AB228013)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-TSG101 antibody (ab228013) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Lane 3:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody (AB228013)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-TSG101 antibody (ab228013) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Lane 3:

Raw264.7 whole cell extracts at 30 µg

Lane 4:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody (AB228013)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-TSG101 antibody (ab228013) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 50 kDa

false

• ELISA

Supplier Data

ELISA - Anti-TSG101 antibody (AB228013)

ELISA detection of TSG101 using ab228013 for capture at a concentration of 5 µg/mL and ab83 for detection at a concentration of 1.5 µg/mL.