Anti-TSG101 抗体 [4A10] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

10% SDS-PAGE

All lanes:

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) at 1/500 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

TSG101 shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 44 kDa

true

• Flow Cyt

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Flow Cytometry - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

Flow Cytometry analysis of THP-1 cells labeling TSG101 with ab83 at 1/25 dilution (red). Unlabelled sample was used as a control (black). A Dylight 488-conjugated secondary antibody was used for FACS analysis.

• Flow Cyt

PubMed

Flow Cytometry - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.

Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1 : 400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.

Image from Kahlert C et al., J Biol Chem. 2014;289(7):3869-75. Fig 1(C).; doi: 10.1074/jbc.C113.532267.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling TSG101 with ab83 at 1/100 dilution. Cytoplasmic staining is observed. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling TSG101 with ab83 at 1/100 dilution. Cytoplasmic staining is observed. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min. (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking : 3% H₂O₂, RT, 30min. Blocking condition : 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30min. Primary antibody incubation : 4°C overnight. Secondary antibody incubation : HRP Kit (Mouse IgG), 1 : 200, RT, 30min. Washing : PBS, 2 x 5 minutes. DAB detection.

• WB

Supplier Data

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

10% SDS-PAGE

All lanes:

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) at 1/500 dilution

Lane 1:

NIH-3T3 whole cell lysate/extract at 30 µg

Lane 2:

JC (mouse mammary adenocarcinoma) whole cell lysate/extract at 30 µg

Lane 3:

BCL-1 whole cell lysate/extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 44 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) at 1/500 dilution

Lane 1:

K562 whole cell lysates at 30 µg

Lane 2:

THP-1 whole cell lysates at 30 µg

Lane 3:

HL-60 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 44 kDa

true

• WB

Supplier Data

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

10% SDS PAGE

Running conditions : 80V for 15min then 140V for 40min

Blocking : 5% non-fat milk in TBST at room temperature for 60min.

Washing conditions : 5 ml TBST, 4 x 5min

Transfer conditions : Semi-dry, 18 V, 60min (NC membrane)

Exposure system : Trident plus Western HRP Substrate

All lanes:

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) at 1/500 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody at 1/500 dilution

Predicted band size: 44 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) at 1/500 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

NIH-3T3 whole cell lysates at 30 µg

Lane 3:

Raw264.7 whole cell lysates at 30 µg

Lane 4:

C2C12 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 44 kDa

false

• WB

Supplier Data

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

All lanes:

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) at 1/500 dilution

Lane 1:

Mouse testis tissue extract at 50 µg

Lane 2:

Mouse pancreas tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 44 kDa

true

• ELISA

Supplier Data

ELISA - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

ELISA detection of TSG101 using for capture at a concentration of 5 µg/mL and for detection at a concentration of 1.5 µg/mL.

• WB

Unknown

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (AB83)

All lanes:

Western blot - Anti-TSG101 antibody [4A10] - BSA and Azide free (ab83) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - A-431 whole cell lysate (<a href='/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Lane 3:

Western blot - Jurkat whole cell lysate (<a href='/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 4:

Western blot - HEK-293 whole cell lysate (<a href='/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 20 µg

Secondary

All lanes:

Donkey polyclonal to Mouse IgG (IRDyeTM 700DX) at 1/10000 dilution

Predicted band size: 44 kDa

Observed band size: 49 kDa

false