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リコンビナントRabMAb

Anti-Tryptophanyl tRNA synthetase/WRS 抗体 [EPR3424] (ab92733)

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Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
  • Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
  • Flow Cytometry (Intracellular) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
  • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424]
    Tryptophanyl tRNA synthetase/WRS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: 293T and HeLa cell lysates, and Human thyroid tissue lysate ICC/IF: MCF7 cells Flow Cyt (intra): HUVEC cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab92733の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

For unpurified use at 1/10000 - 1/100000.
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250.
Flow Cyt (Intra)
1/600.

For unpurified use at 1/1000.
特記事項
WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

For unpurified use at 1/10000 - 1/100000.
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250.
Flow Cyt (Intra)
1/600.

For unpurified use at 1/1000.

ターゲット情報

  • 機能

    Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.

  • 配列類似性

    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.

  • 翻訳後修飾

    Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Gamma 2 antibody
      • GAMMA-2 antibody
      • Gamma2 antibody
      • hWRS antibody
      • IFI 53 antibody
      • IFI53 antibody
      • IFP 53 antibody
      • IFP53 antibody
      • Interferon induced protein 53 antibody
      • Interferon-induced protein 53 antibody
      • SYWC_HUMAN antibody
      • T2-TrpRS antibody
      • TrpRS antibody
      • Tryptophan tRNA ligase, cytoplasmic antibody
      • Tryptophan tRNA ligase 1 cytoplasmic antibody
      • Tryptophan tRNA ligase antibody
      • Tryptophan--tRNA ligase antibody
      • Tryptophanyl tRNA synthetase antibody
      • Tryptophanyl tRNA synthetase cytoplasmic antibody
      • WARS antibody
      • WARS protein antibody
      • WRS antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
      Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
      Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Tryptophanyl tRNA synthetase/WRS with purified ab92733 at 1:50 dilution (10.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
      Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
      All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733) at 1/1000 dilution (Purified)

      Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : Human thyroid lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 53 kDa



      Full-length TrpRS: 54kDa; mini TrpRS: 48kDa; T1 TrpRS: 47kDa; T2 TrpRS: 44kDa. The molecular weights observed are consistent with what has been described in the literature (PMID: 29666190). 

    • Flow Cytometry (Intracellular) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
      Flow Cytometry (Intracellular) - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

      Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) treated with 20ng/ml Interferon gamma for 48 hours cells labeling Tryptophanyl tRNA synthetase/WRS with purified ab92733 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
      IFN gamma induction was reported in literature. (PMID: 31324718)

    • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
      Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

    参考文献 (0)

    ab92733 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab92733 は論文での使用が確認できていません。

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