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AB228724

Anti-Tryptophanyl tRNA synthetase/WRS 抗体

Anti-Tryptophanyl tRNA synthetase/WRS antibody

  • JP Deleterious:医薬用外劇物

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Rabbit Polyclonal Tryptophanyl tRNA synthetase/WRS antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human WARS1.

別名を表示する

IFI53, WARS, WRS, WARS1, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase, IFP53, TrpRS, hWRS

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Paraffin-embedded U87 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Paraffin-embedded C2C12 xenograft tissue stained for Tryptophanyl tRNA synthetase/WRS using ab228724 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Tryptophanyl tRNA synthetase protein at cytoplasm with ab228724 at a 1/85 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)
  • WB

Supplier Data

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (AB228724)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody (ab228724) at 1/10000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

WARS shRNA transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

true

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human WARS1. The exact immunogen used to generate this antibody is proprietary information.

P23381

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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下記製品もご検討ください

AB109213

Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3423]

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0 Reviews
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tryptophanyl tRNA synthetase also known as WRS or TrpRS is an enzyme responsible for attaching tryptophan to its corresponding tRNA during protein synthesis. This enzyme is approximately 53 kDa in mass. It ensures the accurate translation of the genetic code in mRNA into proteins by catalyzing the esterification of tryptophan to the tRNA^Trp. WRS is mostly found in the cytoplasm of cells across various tissues contributing to its role in protein biosynthesis.
Biological function summary

WRS is involved in the important process of protein translation ensuring fidelity in the genetic code transfer from RNA to protein. As part of the aminoacyl-tRNA synthetases family WRS helps in maintaining the overall function and stability of the translational apparatus. It is not typically known to be part of any larger protein complex. In addition to its core function in protein synthesis WRS possesses non-canonical roles related to immune response regulation and angiogenesis.

Pathways

WRS plays key roles in the aminoacyl-tRNA biosynthesis and mTOR signaling pathways. These pathways are essential for regulating protein synthesis and cell growth. In the mTOR pathway WRS's function relates to its interaction with proteins involved in cell growth and proliferation. Its roles within these pathways highlight its influence on cellular homeostasis and protein regulation interacting closely with other synthetases and transcription factors.

WRS has associations with certain cancers and autoimmune disorders. Abnormal expression of WRS is observed in various cancer types reflecting its contribution to dysregulated protein synthesis and cell growth. Additionally WRS has been linked to autoimmune diseases like dermatomyositis where its immune-regulatory roles become compromised. In these conditions WRS's interactions with proteins such as interferons illustrate its importance in the pathophysiology of inflammation and immune response.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction : tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).. Isoform 1. Has no angiostatic activity.. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed : 11773625, PubMed : 11773626, PubMed : 14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed : 14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed : 14630953).. Isoform 2. Has an angiostatic activity.
See full target information WARS1
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保証に関する詳細については利用規約をご確認ください。
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