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リコンビナントRabMAb

Anti-TRRAP 抗体 [EPR14952-81] (ab187166)

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Western blot - Anti-TRRAP antibody [EPR14952-81] (ab187166)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRRAP antibody [EPR14952-81] (ab187166)
  • Anti-TRRAP antibody [EPR14952-81] (ab187166)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14952-81] to TRRAP
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TRRAP antibody [EPR14952-81]
    TRRAP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14952-81] to TRRAP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, Jukat, Molt-4 and K562 cell lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14952-81

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187166の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500.
WB
1/1000 - 1/2000. Detects a band of approximately 438 kDa (predicted molecular weight: 438 kDa).
特記事項
ICC/IF
1/500.
WB
1/1000 - 1/2000. Detects a band of approximately 438 kDa (predicted molecular weight: 438 kDa).

ターゲット情報

  • 関連性

    TRRAP (TRansformation/tRanscription domain Associated Protein) is an adapter protein that is found in a number of multiprotein chromatin complexes with histone acetyltransferase (HAT) activity. These complexes include PCAF, TFTC-HAT, TIP60 HAT, STAGA, and BAF53 complexes. TRRAP is thought to be responsible for the concerted and context-dependent recruitment of these complexes to chromatin during transcription, replication and DNA repair. TRRAP plays a key role in transcriptional activation by c-Myc, p53/TP53, E2F1 and E2F4.

  • 細胞内局在

    Nuclear

  • 参照データベース

    • 別名

      • 350/400 kDa PCAF associated factor antibody
      • PAF 350/400 antibody
      • PAF 400 antibody
      • PAF350/400 antibody
      • PAF400 antibody
      • STAF 40 antibody
      • STAF40 antibody
      • TR AP antibody
      • Tra 1 antibody
      • Tra1 antibody
      • Tra1 homolog antibody
      • Transactivation / transformation domain associated protein antibody
      • Transactivation/transformation domain associated protein antibody
      • Transformation/transcription domain associated protein antibody
      • TRAP antibody
      see all

    画像

    • Western blot - Anti-TRRAP antibody [EPR14952-81] (ab187166)
      Western blot - Anti-TRRAP antibody [EPR14952-81] (ab187166)
      All lanes : Anti-TRRAP antibody [EPR14952-81] (ab187166) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 438 kDa
      Observed band size: 438 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TRRAP antibody [EPR14952-81] (ab187166)
      Immunocytochemistry/ Immunofluorescence - Anti-TRRAP antibody [EPR14952-81] (ab187166)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TRRAP with ab187166 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    • Anti-TRRAP antibody [EPR14952-81] (ab187166)
      Anti-TRRAP antibody [EPR14952-81] (ab187166)

    参考文献 (0)

    ab187166 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187166 は論文での使用が確認できていません。

    レビューと Q&A

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