Anti-TRPV4 抗体 (ab39260)
Key features and details
- Rabbit polyclonal to TRPV4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Horse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TRPV4 antibody
TRPV4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRPV4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Horse
交差が予測される動物種: Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Mouse TRPV4.
Immunogen の所有権に関して (Peptide available as ab39471.) -
ポジティブ･コントロール
- ICC/IF: TKPTS cells
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特記事項
Although some of our customers have had good results in Human and Rat (89% homology with the immunogen), we do not batch test this antibody in these species. Due to the polyclonal nature of this antibody, some batches many not work in Human or Rat.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39260の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (8) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 98 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| IHC-P | (4) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 98 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. -
組織特異性
Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level). -
関連疾患
Brachyolmia 3
Spondylometaphyseal dysplasia Kozlowski type
Metatropic dysplasia
Distal spinal muscular atrophy, congenital non-progressive
Charcot-Marie-Tooth disease 2C
Scapuloperoneal spinal muscular atrophy
Spondyloepiphyseal dysplasia Maroteaux type
Parastremmatic dwarfism
Digital arthropathy-brachydactyly, familial -
配列類似性
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
Contains 3 ANK repeats. -
翻訳後修飾
Phosphorylation results in enhancement of its channel function. -
細胞内局在
Cell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 100066552 Horse
- Entrez Gene: 63873 Mouse
- Entrez Gene: 66026 Rat
- SwissProt: Q9EPK8 Mouse
- SwissProt: Q9ERZ8 Rat
- Unigene: 266450 Mouse
- Unigene: 64508 Rat
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別名
- BCYM3 antibody
- CMT2C antibody
- HMSN2C antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-TRPV4 antibody (ab39260)
ab39260 staining TRPV4 in TKPTS cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab39260 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody (ab39260)IHC image of ab39260 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was then incubated with ab39260, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-TRPV4 antibody (ab39260)Anti-TRPV4 antibody (ab39260) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 25 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 98 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody (ab39260)This image is courtesy of an Abreview submitted by Mr Carl Hobbsab39260 staining of TRPV4 -/- (upper panel) and wildtype (lower panel) adult mouse kidney tissue sections, showing good immunostaining in the wildtype tissue and no immunostaining in the TRPV4 -/- tissue. Formalin/PFA-fixed paraffin-embedded sections of mouse kidney tissue were incubated with ab39260 (1/200) for 2 hours. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0. A biotin-conjugated goat anti-rabbit antibody was used as the secondary. -
Western blot - Anti-TRPV4 antibody (ab39260)Image courtesy of an anonymous Abreview.All lanes : Anti-TRPV4 antibody (ab39260) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC-1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 98 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
This antibody detects a band of ~125 kDa in 4 different mouse cell lines. This is lower than the predicted molecular weight of TRPV4 on SDS-PAGE (100 kDa). However, there are published reports of TRPV4 running at >120 kDa PMID: 16368742 PMID: 12538589, possibly as a result of glycosylation.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (74)
ab39260 は 74 報の論文で使用されています。
- Sun Q et al. Curved Nanofiber Network Induces Cellular Bridge Formation to Promote Stem Cell Mechanotransduction. Adv Sci (Weinh) 10:e2204479 (2023). PubMed: 36382560
- Fu Y et al. Dental Pulp Stem Cell-Derived Exosomes Alleviate Mice Knee Osteoarthritis by Inhibiting TRPV4-Mediated Osteoclast Activation. Int J Mol Sci 24:N/A (2023). PubMed: 36902356
- Ji C et al. TRPV4 regulates β1 integrin-mediated cell-matrix adhesions and collagen remodeling. FASEB J 37:e22946 (2023). PubMed: 37219464
- Zeng ML et al. Inhibition of Transient Receptor Potential Vanilloid 4 (TRPV4) Mitigates Seizures. Neurotherapeutics 19:660-681 (2022). PubMed: 35182379
- Wan H et al. Capsaicin inhibits intestinal Cl- secretion and promotes Na+ absorption by blocking TRPV4 channels in healthy and colitic mice. J Biol Chem 298:101847 (2022). PubMed: 35314195