Anti-TRPV1 抗体 [EPR25057-67] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

This data was developed using ab305299, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde fixed mouse DRG primary neurons permeabilized using 0.1% Triton X-100 labelling TRPV1 with ab305299 (green) followed by ab150081 AlexaFluor^®488 preadsorbed Goat anti-Rabbit secondary. ab78078 anti-beta III Tubulin (mouse mAb) was used as counterstain (magenta) with ab150120 AlexaFluor^®594 Goat anti-Mouse as secondary. Nuclear DNA was labelled with DAPI (shown in blue). Image showing cytoplasmic staining in mouse DRG primary neurons was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

This data was developed using ab305299, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde fixed rat DRG primary neurons permeabilized using 0.1% Triton X-100 labelling TRPV1 with ab305299 (green) followed by ab150081 AlexaFluor^®488 preadsorbed Goat anti-Rabbit secondary. ab78078 anti-beta III Tubulin (mouse mAb) was used as counterstain (magenta) with ab150120 AlexaFluor^®594 Goat anti-Mouse as secondary. Nuclear DNA was labelled with DAPI (shown in blue). Image showing cytoplasmic staining in rat DRG primary neurons was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IP

Supplier Data

Immunoprecipitation - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

This data was developed using ab305299, the same antibody clone in a different buffer formulation.

TRPV1 was immunoprecipitated from 0.35 mg Rat dorsal ganglion tissue lysate 10 μg with ab305299 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305299. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat dorsal ganglion tissue lysate 10 μg

Lane 2 : ab305299 at 1/30 IP in Rat dorsal ganglion tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305299 in Rat dorsal ganglion tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-TRPV1 antibody [EPR25057-67] (<a href='/products/primary-antibodies/trpv1-antibody-epr25057-67-ab305299'>ab305299</a>) at 1/1000 dilution

Lane 1:

Rat dorsal ganglion tissue at 10 µg

Lane 2:

<a href='/products/primary-antibodies/trpv1-antibody-epr25057-67-ab305299'>ab305299</a> at 1/30 IP in rat dorsal ganglion tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 95 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

This data was developed using ab305299, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The molecular weight is consistent to what has been described in literature (PMID : 25395667) Negative control : brain, cerebellum (PMID : 11243859) Samples are non-boiled as boiling may cause protein aggregation. Exposure time : 114 seconds

All lanes:

Western blot - Anti-TRPV1 antibody [EPR25057-67] (<a href='/products/primary-antibodies/trpv1-antibody-epr25057-67-ab305299'>ab305299</a>) at 1/1000 dilution

Lane 1:

Rat dorsal galion tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 40 µg

Lane 3:

Human spinal cord at 20 µg

Lane 4:

Human cerebellum at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 95 kDa

false

Exposure time: 114s

• WB

Lab

Western blot - Anti-TRPV1 antibody [EPR25057-67] - BSA and Azide free (AB305300)

This data was developed using ab305299, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

For Lane1, to minimize protein degradation, cell was lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
Samples are non-boiled as boiling may cause protein aggregation.

All lanes:

Western blot - Anti-TRPV1 antibody [EPR25057-67] (<a href='/products/primary-antibodies/trpv1-antibody-epr25057-67-ab305299'>ab305299</a>) at 1/1000 dilution

Lane 1:

Rat fresh dorsal ganglion tissue lysate at 40 µg

Lane 2:

Rat dorsal ganglion tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,95 kDa

false

Exposure time: 180s