AB228771

Anti-TRPC6 抗体

Anti-TRPC6 antibody

JP Deleterious:医薬用外劇物

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(3 Publications)

Rabbit Polyclonal TRPC6 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRPC6.

別名を表示する

TRP6, TRPC6, Short transient receptor potential channel 6, TrpC6, Transient receptor protein 6, TRP-6

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody (AB228771)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody (AB228771)

Paraffin-embedded mouse cerebellum tissue stained TRPC6 at cytoplasm (green) using ab228771 at 1/500. Red : NF-H, a neuron marker, stained by NF-H antibody at 1/500 dilution. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-TRPC6 antibody (AB228771)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-TRPC6 antibody (ab228771) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell extract at 30 µg

Lane 2:

A549 membrane extract extract at 30 µg

Predicted band size: 106 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human TRPC6. The exact immunogen used to generate this antibody is proprietary information.

Q9Y210

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The TRPC6 also known as Transient Receptor Potential Cation Channel Subfamily C Member 6 functions as a non-selective cation channel permeable to calcium ions. It facilitates calcium entry into cells impacting cellular signaling and homeostasis. TRPC6 has a molecular mass of about 106 kDa. Expression of TRPC6 is broad but is notably significant in tissues such as brain heart lung and skeletal muscle where it influences diverse functions.

Biological function summary

TRPC6 is integral to various cellular processes especially those involving calcium-dependent signaling pathways. It is known to form heteromeric complexes with other TRPC subfamily members such as TRPC3 and TRPC7 which modulates its function. These interactions are important for the regulation of intracellular calcium levels impacting cellular excitability contraction and growth.

Pathways

TRPC6 is involved in the phospholipase C (PLC) pathway and subsequent inositol triphosphate (IP3) signaling. This signaling cascade is vital for the regulation of calcium release from intracellular stores. TRPC6 also has a notable role in the Ras homolog family member A (RhoA) signaling pathway which affects cytoskeletal dynamics and cell motility. Inside these pathways proteins like IP3 receptors and RhoA GTPases are closely related to TRPC6 function.

TRPC6 is associated with conditions like focal segmental glomerulosclerosis (FSGS) and pulmonary hypertension. Mutations in TRPC6 can lead to abnormal regulation of calcium homeostasis in kidney cells contributing to FSGS. Moreover TRPC6 overactivity in pulmonary vascular smooth muscle cells has been implicated in the pathology of pulmonary hypertension. In these conditions TRPC6's interaction with calcineurin and other calcium-dependent signaling proteins plays a critical role in disease progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Forms a receptor-activated non-selective calcium permeant cation channel (PubMed : 19936226, PubMed : 23291369, PubMed : 26892346, PubMed : 9930701). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C (PubMed : 26892346). Seems not to be activated by intracellular calcium store depletion.

See full target information TRPC6

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 17:1302470 PubMed38260021

2024

Characterization of a loss-of-function NSF attachment protein beta mutation in monozygotic triplets affected with epilepsy and autism using cortical neurons from proband-derived and CRISPR-corrected induced pluripotent stem cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Gowher Ali,Kyung Chul Shin,Wesal Habbab,Ghaneya Alkhadairi,Alice AbdelAleem,Fouad A AlShaban,Yongsoo Park,Lawrence W Stanton

Molecular neurobiology 60:7297-7308 PubMed37552395

2023

Deletion of TRPC6, an Autism Risk Gene, Induces Hyperexcitability in Cortical Neurons Derived from Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Chul Shin,Gowher Ali,Houda Yasmine Ali Moussa,Vijay Gupta,Alberto de la Fuente,Hyung-Goo Kim,Lawrence W Stanton,Yongsoo Park

Frontiers in pharmacology 12:730416 PubMed34880749

2021

Regulation of Cytochrome P450 2a5 by and 6,7-Dimethylesculetin in Mouse Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sangsoo Daniel Kim,Larry Morgan,Elyse Hargreaves,Xiaoying Zhang,Zhihui Jiang,Monica Antenos,Ben Li,Gordon M Kirby

View all publications

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Abcam product promise

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Anti-TRPC6 抗体

Anti-TRPC6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody (AB228771)

Western blot - Anti-TRPC6 antibody (AB228771)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise