AB105845

Anti-TRPC6 抗体 [3F2.H10.F2]

Name: Anti-TRPC6 抗体 [3F2.H10.F2] (ab105845) | Abcam
Brand: Abcam Limited
SKU: AB105845
Price: 72000 JPY
Availability: InStock
Rating: 5 (3 reviews)

Anti-TRPC6 antibody [3F2.H10.F2]

(3 Reviews)

(4 Publications)

Mouse Monoclonal TRPC6 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications.

別名を表示する

TRP6, TRPC6, Short transient receptor potential channel 6, TrpC6, Transient receptor protein 6, TRP-6

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody [3F2.H10.F2] (AB105845)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody [3F2.H10.F2] (AB105845)

ab105845 at 2.5 μg/ml staining TRPC6 in Human adrenal (cortex) tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows strong staining with minimal background staining. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Supplier Data

Western blot - Anti-TRPC6 antibody [3F2.H10.F2] (AB105845)

Western blot analysis of ab105845 with mouse kidney (10ug) at the dilution 1/1000.Secondary antibody (DyLight™ 649 donkey anti-mouse) was used at the dilution 1/20000

All lanes:

Western blot - Anti-TRPC6 antibody [3F2.H10.F2] (ab105845)

Predicted band size: 106 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

3F2.H10.F2

アイソタイプ

IgG1

キャリアフリー

交差種

Mouse, Human

アプリケーション

IHC-P, WB, ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Purified from concentrated tissue culture supernate by Protein A chromatography.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The TRPC6 also known as Transient Receptor Potential Cation Channel Subfamily C Member 6 functions as a non-selective cation channel permeable to calcium ions. It facilitates calcium entry into cells impacting cellular signaling and homeostasis. TRPC6 has a molecular mass of about 106 kDa. Expression of TRPC6 is broad but is notably significant in tissues such as brain heart lung and skeletal muscle where it influences diverse functions.

Biological function summary

TRPC6 is integral to various cellular processes especially those involving calcium-dependent signaling pathways. It is known to form heteromeric complexes with other TRPC subfamily members such as TRPC3 and TRPC7 which modulates its function. These interactions are important for the regulation of intracellular calcium levels impacting cellular excitability contraction and growth.

Pathways

TRPC6 is involved in the phospholipase C (PLC) pathway and subsequent inositol triphosphate (IP3) signaling. This signaling cascade is vital for the regulation of calcium release from intracellular stores. TRPC6 also has a notable role in the Ras homolog family member A (RhoA) signaling pathway which affects cytoskeletal dynamics and cell motility. Inside these pathways proteins like IP3 receptors and RhoA GTPases are closely related to TRPC6 function.

TRPC6 is associated with conditions like focal segmental glomerulosclerosis (FSGS) and pulmonary hypertension. Mutations in TRPC6 can lead to abnormal regulation of calcium homeostasis in kidney cells contributing to FSGS. Moreover TRPC6 overactivity in pulmonary vascular smooth muscle cells has been implicated in the pathology of pulmonary hypertension. In these conditions TRPC6's interaction with calcineurin and other calcium-dependent signaling proteins plays a critical role in disease progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Forms a receptor-activated non-selective calcium permeant cation channel (PubMed : 19936226, PubMed : 23291369, PubMed : 26892346, PubMed : 9930701). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C (PubMed : 26892346). Does not seem to be activated by intracellular calcium store depletion.

See full target information TRPC6

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:516-526 PubMed40681898

2025

Pathology-oriented multiplexing enables integrative disease mapping.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Kuehl,Yusuke Okabayashi,Milagros N Wong,Lukas Gernhold,Gabriele Gut,Nico Kaiser,Maria Schwerk,Stefanie K Gräfe,Frank Y Ma,Jovan Tanevski,Philipp S L Schäfer,Sam Mezher,Jacobo Sarabia Del Castillo,Thiago Goldbeck-Strieder,Olga Zolotareva,Michael Hartung,Fernando M Delgado Chaves,Lukas Klinkert,Ann-Christin Gnirck,Marc Spehr,David Fleck,Mehdi Joodaki,Victor Parra,Mina Shaigan,Martin Diebold,Marco Prinz,Jennifer Kranz,Johan M Kux,Fabian Braun,Oliver Kretz,Hui Wu,Florian Grahammer,Sven Heins,Marina Zimmermann,Fabian Haas,Dominik Kylies,Nicola Wanner,Jan Czogalla,Bernhard Dumoulin,Nikolay Zolotarev,Maja Lindenmeyer,Pall Karlson,Jens R Nyengaard,Marcial Sebode,Sören Weidemann,Thorsten Wiech,Hermann-Josef Groene,Nicola M Tomas,Catherine Meyer-Schwesinger,Christoph Kuppe,Rafael Kramann,Alexandre Karras,Patrick Bruneval,Pierre-Louis Tharaux,Diego Pastene,Benito Yard,Jennifer A Schaub,Phillip J McCown,Laura Pyle,Ye Ji Choi,Takashi Yokoo,Jan Baumbach,Pablo J Sáez,Ivan Costa,Jan-Eric Turner,Jeffrey B Hodgin,Julio Saez-Rodriguez,Tobias B Huber,Petter Bjornstad,Matthias Kretzler,Olivia Lenoir,David J Nikolic-Paterson,Lucas Pelkmans,Stefan Bonn,Victor G Puelles

Renal failure 46:2431150 PubMed39566913

2024

TRPC6 knockdown-mediated ERK1/2 inactivation alleviates podocyte injury in minimal change disease via upregulating Lon peptidase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Ma,Liling Ren,Qin Su,Xiuyi Lv,Min Sun,Yunbo Wei,Lili Dai,Xueyan Bian

Experimental and therapeutic medicine 26:576 PubMed38023364

2023

Downregulation of TRPC6 regulates ERK1/2 to prevent sublytic C5b‑9 complement complex‑induced podocyte injury through activating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Youfu Fang,Jing Liu

Antioxidants (Basel, Switzerland) 11: PubMed36358552

2022

OR2AT4, an Ectopic Olfactory Receptor, Suppresses Oxidative Stress-Induced Senescence in Human Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Sun Kim,Ha Lim Lee,Ji Hyun Jeong,Ye Eun Yoon,In-Ryeong Lee,Ji Min Kim,Chunyan Wu,Sung-Joon Lee

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRPC6 抗体 [3F2.H10.F2]

Anti-TRPC6 antibody [3F2.H10.F2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody [3F2.H10.F2] (AB105845)

Western blot - Anti-TRPC6 antibody [3F2.H10.F2] (AB105845)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise