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リコンビナントRabMAb

Anti-TRPC3 抗体 [EPR25056-56] (BSA and Azide free) (ab300143)

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Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
  • Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
  • Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25056-56] to TRPC3 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free)
    TRPC3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25056-56] to TRPC3 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Tissue lysates mouse and rat: cerebellum, brain. IHC-P: Mouse cerebellum, rat cerebellum, HEK-293T transfected with a TRPC3 expression vector.

  • 特記事項

    Ab300143 is a carrier free version of ab300142. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300143の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.
IHC-P
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 96 kDa.
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-Fr or IP.

ターゲット情報

  • 関連性

    TRPC channels form the subfamily of channels in human most closely related to drosophila TRP channels. Structurally, this family shares a number of similar characteristics. At the proximal C-terminus of this sub-family is a TRP box motif containing the invariant EWKFAR sequence and between 3 and 4 ankyrin repeats near the N-terminus. These channels are non-selectively permeable to cations, with a selectivity of calcium over sodium variable among the different members. Many of TRPC channel subunits are able to coassemble.

  • 細胞内局在

    Cell Membrane; multi-pass membrane protein

  • 参照データベース

    • 別名

      • Htrp 3 antibody
      • Htrp3 antibody
      • MGC124333 antibody
      • Mtrp3 antibody
      • Receptor activated cation channel TRP3 antibody
      • Short transient receptor potential channel 3 antibody
      • Transient receptor potential cation channel subfamily C member 3 antibody
      • transient receptor potential cation channel subfamily C member 3 variant c antibody
      • Transient receptor potential channel 3 antibody
      • TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody
      • Transient receptor protein 3 antibody
      • Trp related protein 3 antibody
      • TRP3 antibody
      • TrpC3 antibody
      • Trrp3 antibody
      see all

    画像

    • Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      All lanes : Anti-TRPC3 antibody [EPR25056-56] (ab300142) at 1/1000 dilution

      Lane 1 : HEK-293 transfected with mouse TRPC3 (WT) expression vector containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293 transfected with mouse TRPC7 (WT) expression vector containing a myc-His-tag®, whole cell lysate
      Lane 3 : HEK-293 transfected with mouse TRPC6 (WT) expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 96 kDa
      Observed band size: 105 kDa why is the actual band size different from the predicted?


      Exposure time: 6 seconds


      This data was developed using ab300142, the same antibody clone in a different buffer formulation. 

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      Western blot - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      All lanes : Anti-TRPC3 antibody [EPR25056-56] (ab300142) at 1/1000 dilution

      Lane 1 : Mouse cerebellum tissue lysate
      Lane 2 : Mouse brain tissue lysate
      Lane 3 : Mouse lung tissue lysate (negative control)
      Lane 4 : Rat cerebellum tissue lysate
      Lane 5 : Rat brain tissue lysate
      Lane 6 : Rat lung tissue lysate (negative control)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 96 kDa
      Observed band size: 105 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab300142, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The molecular weight is consistent to literature (PMID: 15834157)
      Low express tissue: lung (PMID:21931279)
      This blot was developed using a higher sensitivity ECL substrate.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

      This data was developed using ab300142, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labelling TRPC3 with ab300142 at 1/100 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebellum tissue. The section was incubated with ab300142 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

      This data was developed using ab300142, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labelling TRPC3 with ab300142 at 1/100 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebellum tissue. The section was incubated with ab300142 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

      This data was developed using ab300142, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T cells labeling TRPC3 with ab300142 at 1/500 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on (A) HEK-293T transfected with a TRPC3 expression vector; nearly no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300142 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

      This data was developed using ab300142, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labelling TRPC3 with ab300142 at 1/100 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab300142 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)
      Anti-TRPC3 antibody [EPR25056-56] (BSA and Azide free) (AB300143)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300143 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300143 は論文での使用が確認できていません。

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