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リコンビナントRabMAb

Anti-TRP1 抗体 [EPR13063] - BSA and Azide free (ab186929)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
  • Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
  • Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13063] to TRP1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

製品の概要

  • 製品名

    Anti-TRP1 antibody [EPR13063] - BSA and Azide free
    TRP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13063] to TRP1 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab186929 is the carrier-free version of ab178676.

    This antibody is unsuitable for IHC-P on mouse samples.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186929の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. 
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. 
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.

ターゲット情報

  • 機能

    Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.

  • 組織特異性

    Pigment cells.

  • パスウェイ

    Pigment biosynthesis; melanin biosynthesis.

  • 関連疾患

    Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.

  • 配列類似性

    Belongs to the tyrosinase family.

  • 細胞内局在

    Melanosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 5 antibody
      • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
      • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
      • b-PROTEIN antibody
      • CAS2 antibody
      • Catalase B antibody
      • CATB antibody
      • DHICA oxidase antibody
      • Glycoprotein 75 antibody
      • GP75 antibody
      • Melanoma antigen gp75 antibody
      • OCA3 antibody
      • TRP antibody
      • TRP-1 antibody
      • TRP1 antibody
      • Tyrosinase related protein 1 antibody
      • Tyrosinase-related protein 1 antibody
      • TYRP antibody
      • TYRP1 antibody
      • TYRP1_HUMAN antibody
      • TYRRP antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
      Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)

      Immunocytochemistry/ Immunofluorescence analysis of B16-F10 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1/100 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling TRP1 with purified ab178676 at 1/1000 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

    • Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
      Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)

      Intracellular Flow Cytometry analysis of B16-F0 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1/40 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

    • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
      Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)

      Immunofluorescent analysis of B16-F0 cells labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178676).

    • Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)
      Anti-TRP1 antibody [EPR13063] - BSA and Azide free (ab186929)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab186929 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186929 は 1 報の論文で使用されています。

    • Rachmin I  et al. Stress-associated ectopic differentiation of melanocyte stem cells and ORS amelanotic melanocytes in an ex vivo human hair follicle model. Exp Dermatol 30:578-587 (2021). PubMed: 33598985

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