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リコンビナントRabMAb

Anti-TRP1 抗体 [EPR13063] (ab178676)

Submit a review Submit a question References (12)
Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)
  • Anti-TRP1 antibody [EPR13063] (ab178676)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13063] to TRP1
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-TRP1 antibody [EPR13063]
    TRP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13063] to TRP1

  • 由来種

    Rabbit

  • 特異性

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human melanoma lysate; B16-F0 cell lysate; Human skin tissue; B16-F0 cells. WB: MeWo, B16-F0 and SK-MEL-28 cell lysates

  • 特記事項

    This antibody is unsuitable for IHC-P on mouse samples.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab178676の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/40.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For the unpurified format use at 1/100 - 1/250 dilution.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
WB
1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
ICC/IF
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/40.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For the unpurified format use at 1/100 - 1/250 dilution.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
WB
1/10000 - 1/50000. Predicted molecular weight: 61 kDa.
ICC/IF
1/100 - 1/250.

ターゲット情報

  • 機能

    Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.

  • 組織特異性

    Pigment cells.

  • パスウェイ

    Pigment biosynthesis; melanin biosynthesis.

  • 関連疾患

    Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.

  • 配列類似性

    Belongs to the tyrosinase family.

  • 細胞内局在

    Melanosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 5 antibody
      • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
      • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
      • b-PROTEIN antibody
      • CAS2 antibody
      • Catalase B antibody
      • CATB antibody
      • DHICA oxidase antibody
      • Glycoprotein 75 antibody
      • GP75 antibody
      • Melanoma antigen gp75 antibody
      • OCA3 antibody
      • TRP antibody
      • TRP-1 antibody
      • TRP1 antibody
      • Tyrosinase related protein 1 antibody
      • Tyrosinase-related protein 1 antibody
      • TYRP antibody
      • TYRP1 antibody
      • TYRP1_HUMAN antibody
      • TYRRP antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] (ab178676)
      Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] (ab178676)

      Intracellular Flow Cytometry analysis of B16-F0 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1/40 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      All lanes : Anti-TRP1 antibody [EPR13063] (ab178676) at 1/50000 dilution (Purified)

      Lane 1 : MeWo (Human malignant melanoma fibroblast) whole cell lysates
      Lane 2 : B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 61 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      The observed molecular weight is consistent with what have been described in literatures PMID: 24586946 and 27690000.

    • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution (Purified) + SK-MEL-28 (Human malignant melanoma ) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 61 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      The observed molecular weight is consistent with what have been described in literatures PMID: 24586946 and 27690000.

    • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)
      Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)
      Immunocytochemistry/ Immunofluorescence analysis of B16-F10 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1:100 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling TRP1 with purified ab178676 at 1:1000 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution (unpurified) + Human melanoma lysate at 10 µg

      Predicted band size: 61 kDa

    • Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)
      Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution (unpurified) + B16-F0 cell lysate at 10 µg

      Predicted band size: 61 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)
      Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)

      Immunofluorescent analysis of B16-F0 cells labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

    • Anti-TRP1 antibody [EPR13063] (ab178676)
      Anti-TRP1 antibody [EPR13063] (ab178676)

    参考文献 (12)

    ab178676 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab178676 は 12 報の論文で使用されています。

    • Hu SH  et al. sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes. Oxid Med Cell Longev 2022:1856830 (2022). PubMed: 35186181
    • Villareal MO  et al. Molecular Analysis of the Melanogenesis Inhibitory Effect of Saponins-Rich Fraction of Argania spinosa Leaves Extract. Molecules 27:N/A (2022). PubMed: 36235295
    • Zou DP  et al. SFRP5 inhibits melanin synthesis of melanocytes in vitiligo by suppressing the Wnt/β-catenin signaling. Genes Dis 8:677-688 (2021). PubMed: 34291139
    • Tang X  et al. Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion. Pharmaceutics 13:N/A (2021). PubMed: 34452146
    • Qiao Z  et al. Dysfunction of ATG7-dependent autophagy dysregulates the antioxidant response and contributes to oxidative stress-induced biological impairments in human epidermal melanocytes. Cell Death Discov 6:31 (2020). PubMed: 32377394
    View all Publications for this product

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