Anti-Tropomyosin 抗体 [TM-311] (ab7785)
![Western blot - Anti-Tropomyosin antibody [TM-311] (ab7785)](https://www.abcam.co.jp/ps/products/7/ab7785/Images/ab7785-13243-anti-tropomyosin-antibody-tm-311-western-blot.jpg "Western blot - Anti-Tropomyosin antibody [TM-311] (ab7785)")
Key features and details
- Mouse monoclonal [TM-311] to Tropomyosin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Tropomyosin antibody [TM-311] -
製品の詳細
Mouse monoclonal [TM-311] to Tropomyosin -
由来種
Mouse -
特異性
This antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Purified chicken gizzard tropomyosin.
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ポジティブ･コントロール
- WB: Human heart tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
TM-311 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa).
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| 特記事項 |
|---|
|
WB
1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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関連性
Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 7168 Human
- Entrez Gene: 7169 Human
- Entrez Gene: 7170 Human
- Omim: 190990 Human
- Omim: 191010 Human
- Omim: 191030 Human
- SwissProt: P06753 Human
- SwissProt: P07951 Human
see all -
別名
- Alpha tropomyosin antibody
- Beta tropomyosin antibody
- TM30nm antibody
see all
画像
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Western blot - Anti-Tropomyosin antibody [TM-311] (ab7785)Anti-Tropomyosin antibody [TM-311] (ab7785) at 1/200 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab7785 は 16 報の論文で使用されています。
- Sultan A et al. Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat. Life (Basel) 12:N/A (2022). PubMed: 36013400
- Zhang W et al. Skullcapflavone I inhibits proliferation of human colorectal cancer cells via down-regulation of miR-107 expression. Neoplasma 66:203-210 (2019). PubMed: 30509103
- Shibata T et al. Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity. Mech Ageing Dev 171:24-30 (2018). IHC-P ; Mouse . PubMed: 29510160
- Sui Z et al. Stabilization of F-actin by tropomyosin isoforms regulates the morphology and mechanical behavior of red blood cells. Mol Biol Cell 28:2531-2542 (2017). PubMed: 28720661
- Ijaz F et al. Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires Ttll4. Mol Biol Cell 28:535-544 (2017). PubMed: 27974641