AB180176

Anti-Tropomyosin 2 抗体 [EPR12846] - C-terminal

Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Tropomyosin 2 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

TMSB, TPM2, Tropomyosin beta chain, Beta-tropomyosin, Tropomyosin-2

3 Images

Supplier Data

Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (AB180176)

Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate labeling Tropomyosin 2 using ab180176 at 1/10.

All lanes:

Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176)

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (AB180176)

All lanes:

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HT-1080 cell lysate at 10 µg

Lane 4:

Fetal muscle lysate at 10 µg

Secondary

All lanes:

Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (AB180176)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (ab180176) at 1/1000 dilution

Lane 1:

MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)

Lane 2:

MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)

Lane 3:

MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)

Lane 4:

GST-tagged Recombinant Human TPM4 protein (Full length)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 42 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR12846

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin 2 (TPM2) is a protein that plays an important mechanical role in muscle function. It is a component of the actin filament system weighing approximately 32.7 kDa. TPM2 interacts with actin to stabilize the filament structures which are essential in muscle contraction processes. This protein is expressed mainly in muscle tissues including skeletal and cardiac muscles but is also present in other tissues. Its stability and interaction with actin filaments make it significant in maintaining sarcomere structure.

Biological function summary

TPM2 ensures proper muscle contraction by regulating the interaction between actin and myosin two key components in muscle movement. It forms a complex with actin and troponin a critical protein that regulates muscle contraction through calcium ion binding. This complex facilitates muscle fiber contraction by exposing the binding sites for myosin on actin filaments. The presence of TPM2 in the complex is essential for the contraction mechanism allowing precise control of muscle movements.

Pathways

TPM2 acts in the regulation of muscle contraction and actin cytoskeleton signaling pathways. In the muscle contraction pathway TPM2 interacts closely with tropomyosin 1 (TPM1) and the myosin motor protein playing a regulatory role. It is involved in the actin cytoskeleton signaling pathway where it helps organize actin filaments necessary for various cellular activities. Through these pathways TPM2 integrates signals essential for muscle activity and cellular architecture maintenance.

TPM2 mutations have a notable connection to conditions such as nemaline myopathy and distal arthrogryposis. Nemaline myopathy is characterized by muscle weakness and it arises due to defects in sarcomere proteins with TPM2 being a significant player. Additionally changes in TPM2 can associate with contractures and joint deformities in distal arthrogryposis. The protein connects to cellular disorders through its interactions with troponin I (TNNI2) and other structural sarcomere components highlighting its critical role in muscle integrity and function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.

See full target information TPM2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 27:1112-1119 PubMed30019485

2018

Rapamycin ameliorates psoriasis by regulating the expression and methylation levels of tropomyosin via ERK1/2 and mTOR pathways in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Minhong Gao,Xiaoqing Si

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tropomyosin 2 抗体 [EPR12846] - C-terminal

Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal

Immunoprecipitation - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (AB180176)

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (AB180176)

Western blot - Anti-Tropomyosin 2 antibody [EPR12846] - C-terminal (AB180176)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise