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リコンビナントRabMAb

Anti-Tropomyosin 1 (alpha) 抗体 [EPR5158] (ab109505)

Submit a review Submit a question References (3)
Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
  • Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
  • Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5158] to Tropomyosin 1 (alpha)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Tropomyosin 1 (alpha) antibody [EPR5158]
    Tropomyosin 1 (alpha) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5158] to Tropomyosin 1 (alpha)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human heart, Mouse heart, and Rat heart tissue lysates, and Recombinant Human TMP1 protein. IHC-P: Human cardiac muscle, Rat cardiac muscle, Mouse cardiac muscle, and Human prostatic hyperplasia tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109505の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

ターゲット情報

  • 機能

    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

  • 組織特異性

    Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

  • 関連疾患

    Defects in TPM1 are the cause of cardiomyopathy familial hypertrophic type 3 (CMH3) [MIM:115196]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TPM1 are the cause of cardiomyopathy dilated type 1Y (CMD1Y) [MIM:611878]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

  • 配列類似性

    Belongs to the tropomyosin family.

  • ドメイン

    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

  • 細胞内局在

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AA986836 antibody
    • AI854628 antibody
    • Alpha tropomyosin antibody
    • alpha-TM antibody
    • Alpha-tropomyosin antibody
    • C15orf13 antibody
    • cardiomyopathy, hypertrophic 3 antibody
    • CMD1Y antibody
    • CMH3 antibody
    • HTM alpha antibody
    • HTM-alpha antibody
    • OTTHUMP00000163688 antibody
    • sarcomeric tropomyosin kappa antibody
    • TM2 antibody
    • Tmpa antibody
    • TMSA antibody
    • Tpm-1 antibody
    • TPM1 antibody
    • TPM1_HUMAN antibody
    • tropomyosin 1 (alpha) antibody
    • tropomyosin 1 (alpha) isoform 1 antibody
    • tropomyosin 1 (alpha) isoform 2 antibody
    • tropomyosin 1 (alpha) isoform 3 antibody
    • tropomyosin 1 (alpha) isoform 4 antibody
    • tropomyosin 1 (alpha) isoform 5 antibody
    • tropomyosin 1 (alpha) isoform 6 antibody
    • tropomyosin 1 (alpha) isoform 7 antibody
    • Tropomyosin 1 antibody
    • Tropomyosin alpha 1 chain antibody
    • Tropomyosin alpha-1 chain antibody
    • Tropomyosin, skeletal muscle alpha antibody
    • Tropomyosin-1 antibody
    see all

画像

  • Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    All lanes : Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505) at 1/1000 dilution (Purified)

    Lane 1 : Human heart lysate at 15 µg
    Lane 2 : Mouse heart lysate at 20 µg
    Lane 3 : Rat heart lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    Western blot - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    All lanes : Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505) at 1/1000 dilution

    Lane 1 : MYC and DDK-tagged Recombinant Human TPM1 protein (Full length)
    Lane 2 : MYC and DDK-tagged Recombinant Human TPM2 protein (Full length)
    Lane 3 : MYC and DDK-tagged Recombinant Human TPM3 protein (Full length)
    Lane 4 : GST-tagged Recombinant Human TPM4 protein (Full length)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling Tropomyosin 1 (alpha) with purified ab109505 at 1:600 (0.343 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)
    Anti-Tropomyosin 1 (alpha) antibody [EPR5158] (ab109505)

参考文献 (3)

ab109505 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109505 は 3 報の論文で使用されています。

  • Cisneros E  et al. Identification of Potential Visceral Pain Biomarkers in Colon Exudates from Mice with Experimental Colitis: An Exploratory In Vitro Study. J Pain 24:874-887 (2023). PubMed: 36638875
  • Yoo S  et al. INO80 Is Required for the Cell Cycle Control, Survival, and Differentiation of Mouse ESCs by Transcriptional Regulation. Int J Mol Sci 23:N/A (2022). PubMed: 36499727
  • Pan H  et al. Tropomyosin-1 acts as a potential tumor suppressor in human oral squamous cell carcinoma. PLoS One 12:e0168900 (2017). WB . PubMed: 28182650

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