AB227689

Anti-TROP2 抗体 [SP293] - C-terminal

Anti-TROP2 antibody [SP293] - C-terminal

BOND RX™ Validated
RabMAb
Recombinant
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(6 Publications)

Anti-TROP2 antibody [SP293] - C-terminal (ab227689) is a rabbit monoclonal antibody detecting TROP2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

GA733-1, M1S1, TROP2, TACSTD2, Tumor-associated calcium signal transducer 2, Cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1, Pancreatic carcinoma marker protein GA733-1

15 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human skin tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human tonsil tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human ovarian adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human prostate tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human esophagus tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma cell line) cells, labeling TROP2 with ab227689 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Formalin-fixed paraffin-embedded human bladder tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling TROP2 with ab227689 at 1/50 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 minutes. The section was incubated with ab227689 for 10 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. Membranous staining on the mouse skin. Performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Lab

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Blocking/Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689) at 1/100 dilution

Lane 1:

bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg

Lane 2:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

LLC1 (mouse lung carcinoma) whole cell lysate at 20 µg

Lane 4:

B16F10 (Mouse skin melanoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 36 kDa

Observed band size: 37 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

All lanes:

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (ab227689) at 1/100 dilution

All lanes:

SK-BR-3 (human mammary gland adenocarcinoma cell line) cell lysate

Predicted band size: 36 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP293

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

Flow Cyt (Intra), IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature." }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature." } } }

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製品の詳細

What is this antibody validated in?
Anti-TROP2 antibody [SP293] - C-terminal (ab227689) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of TROP2?
Anti-TROP2 [SP293] - C-terminal (ab227689) specifically detects a band for TROP2 (UniProt: P09758) at a molecular weight of 36kDa.

Collaboration
Anti-TROP2 [SP293] - C-terminal (ab227689) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products
We have a range of other formats of antibody clone [SP293] also available for your convenience: ab227689, Carrier free - ab272014, PE - ab306220, APC - ab306221, HRP - ab306222, Alkaline Phosphatase - ab309002, Alexa Fluor® 488 - ab309944, Alexa Fluor® 647 - ab310311, Alexa Fluor® 594 - ab310775, Alexa Fluor® 555 - ab312301, Alexa Fluor® 568 - ab312797, Alexa Fluor® 750 - ab321453

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

精製に関する特記事項

Purified from TCS by protein A/G.

バッファー組成

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TROP2 also known as TACSTD2 or tumor-associated calcium signal transducer 2 is a single-pass type I membrane glycoprotein. This protein is about 35 kDa in mass and primarily expresses in epithelial tissues. It acts as a transmembrane receptor that plays a role in signal transduction due to its domain structure binding and activation processes on the cell surface. TROP2 exhibits high expression in several types of tumors making it a potential target for therapeutic antibodies like anti-TROP2 anti trop2 or anti-TROP-2.

Biological function summary

TROP2 mediates calcium signaling and cell proliferation. It functions by being a part of a signaling complex on the cell membrane which affects multiple cellular activities such as cell growth motility and survival. Its role in maintaining the integrity of the epithelial cell layer also shows TROP2's significance in normal tissue homeostasis. The interaction of TROP2 with the extracellular matrix and other cell surface proteins support the structural integrity and communication between cells.

Pathways

TROP2 plays a central role in the PI3K/AKT and MAPK signaling pathways that regulate cell growth and survival. Within these pathways TROP2 works in concert with proteins like ERK and AKT important for transmitting extracellular signals into appropriate cellular responses. These pathways are key regulators of many physiological processes including cell cycle progression and apoptosis prevention.

TROP2 is strongly associated with several types of cancers particularly epithelial cancers like breast and pancreatic cancer. Overexpression of TROP2 in tumors often correlates with poor prognosis and aggressive disease phenotypes. Interaction between TROP2 and proteins like EGFR in cancer cell signaling pathways amplifies tumor progression and metastasis. Understanding these associations makes TROP2 a significant target in developing therapeutic strategies for cancer treatment.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May function as a growth factor receptor.

See full target information TACSTD2

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30313 PubMed40830367

2025

Three-dimensional analysis of intraepidermal nerve fibres and Langerhans cells in keloids with a focus on pruritus.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Matsuzoe,Koh-Ei Toyoshima,Miki Takase,Ayako Tsuchiya,Mika Ikeda,Motoko Naitoh,Kazuya Kataoka,Tomoya Kawabata,Miho Ogawa,Naoki Morimoto,Takashi Tsuji

Cancer medicine 14:e70615 PubMed40059728

2025

Prognostic Value of Trop-2 Expression in Nonmetastatic Triple-Negative Breast Cancer and Correlation With Emerging Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

William Jacot,Marie-Christine Chateau,Simon Thezenas,Séverine Guiu,Nelly Firmin,Virginie Lafont,Gwendal Lazennec,Charles Theillet,Florence Boissière-Michot

Current genomics 24:129-131 PubMed38178982

2024

Trop-2 as an Actionable Biomarker in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Cursano,Emanuele Frigo,Elham Sajjadi,Mariia Ivanova,Konstantinos Venetis,Elena Guerini-Rocco,Carmen Criscitiello,Giuseppe Curigliano,Nicola Fusco

Scientific reports 12:22498 PubMed36577919

2022

Correlation of TROP-2 expression with clinical-pathological characteristics and outcome in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hava Izci,Kevin Punie,Lise Waumans,Annouschka Laenen,Hans Wildiers,Freija Verdoodt,Christine Desmedt,Jan Ardui,Ann Smeets,Sileny N Han,Ines Nevelsteen,Patrick Neven,Giuseppe Floris

Scientific reports 12:9583 PubMed35688908

2022

TACSTD2 upregulation is an early reaction to lung infection.

Applications

Unspecified application

Species

Unspecified reactive species

Sára Lenárt,Peter Lenárt,Lucia Knopfová,Hana Kotasová,Vendula Pelková,Veronika Sedláková,Ondřej Vacek,Jana Pokludová,Vladimír Čan,Jan Šmarda,Karel Souček,Aleš Hampl,Petr Beneš

Oncology reports 40:759-766 PubMed29901160

2018

Trop2 is a potential biomarker for the promotion of EMT in human breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhao,Xingwang Kuai,Xueyi Zhou,Lizhou Jia,Jinsong Wang,Xiaobing Yang,Zhidan Tian,Xiaolei Wang,Qian Lv,Bin Wang,Youcai Zhao,Wenbin Huang

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-TROP2 抗体 [SP293] - C-terminal

Anti-TROP2 antibody [SP293] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Flow Cytometry (Intracellular) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

Western blot - Anti-TROP2 antibody [SP293] - C-terminal (AB227689)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

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