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リコンビナントRabMAb

Anti-TrkA 抗体 [EPR25641-50] (ab302524)

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Western blot - Anti-TrkA antibody [EPR25641-50] (AB302524)
  • Western blot - Anti-TrkA antibody [EPR25641-50] (AB302524)
  • Immunocytochemistry/ Immunofluorescence - Anti-TrkA antibody [EPR25641-50] (ab302524)
  • Immunoprecipitation - Anti-TrkA antibody [EPR25641-50] (ab302524)
  • Anti-TrkA antibody [EPR25641-50] (AB302524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25641-50] to TrkA
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TrkA antibody [EPR25641-50]
    TrkA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25641-50] to TrkA

  • 由来種

    Rabbit

  • 特異性

    This antibody does not cross-react with human TrkB and TrkC.

  • アプリケーション

    適用あり: ICC/IF, WB, IPmore details
    適用なし: Flow Cyt (Intra),IHC-Fr or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: TF-1, SH-SY5Y, 293T transfected with human TrkA expression vector containing a myc-His-tag® whole cell lysates. ICC/IF: TF-1 cell line. IP: TF-1 whole cell lysate

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302524の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50.
WB
1/1000. Predicted molecular weight: 87 kDa.
IP
1/30.
特記事項
ICC/IF
1/50.
WB
1/1000. Predicted molecular weight: 87 kDa.
IP
1/30.
追加情報
Is unsuitable for Flow Cyt (Intra),IHC-Fr or IHC-P.

ターゲット情報

  • 機能

    Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
    Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.

  • 組織特異性

    Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.

  • 関連疾患

    Congenital insensitivity to pain with anhidrosis
    Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241).

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.

  • ドメイン

    The transmembrane domain mediates interaction with KIDINS220.
    The extracellular domain mediates interaction with NGFR.

  • 翻訳後修飾

    Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
    N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
    Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.

  • 細胞内局在

    Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).
  • Information by UniProt

  • 参照データベース

    • 別名

      • gp140trk antibody
      • High affinity nerve growth factor receptor antibody
      • High affinity nerve growth factor receptor precursor antibody
      • MTC antibody
      • Neurotrophic tyrosine kinase receptor type 1 antibody
      • NTRK1 antibody
      • NTRK1_HUMAN antibody
      • Oncogene TRK antibody
      • p14-TrkA antibody
      • p140 TrkA antibody
      • p140-TrkA antibody
      • Slow nerve growth antibody
      • Trk A antibody
      • TRK antibody
      • Trk-A antibody
      • TRK1 antibody
      • TRK1-transforming tyrosine kinase protein antibody
      • Tropomyosin-related kinase A antibody
      • Tyrosine kinase receptor A antibody
      • Tyrosine kinase receptor antibody
      see all

    画像

    • Western blot - Anti-TrkA antibody [EPR25641-50] (AB302524)
      Western blot - Anti-TrkA antibody [EPR25641-50] (AB302524)
      All lanes : Anti-TrkA antibody [EPR25641-50] (ab302524) at 1/1000 dilution

      Lane 1 : TF-1 (human Erythroleukemia erythroblast), whole cell lysate
      Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
      Lane 3 : 293T (human embryonic kidney epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 87 kDa
      Observed band size: 100,40 kDa why is the actual band size different from the predicted?


      Exposure time: 70 seconds


      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19330021, 8636219).

      Negative control: 293T (PMID: 10523865, 11251075)

      The blot of lane 2 + lane 3 was developed using a high sensitivity ECL substrate.

    • Western blot - Anti-TrkA antibody [EPR25641-50] (AB302524)
      Western blot - Anti-TrkA antibody [EPR25641-50] (AB302524)
      All lanes : Anti-TrkA antibody [EPR25641-50] (ab302524) at 1/1000 dilution

      Lane 1 : 293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
      Lane 2 : 293T transfected with human TrkA expression vector containing a myc-His-tag®, whole cell lysate
      Lane 3 : 293T transfected with human TrkB expression vector containing a myc-His-tag®, whole cell lysate
      Lane 4 : 293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 87 kDa
      Observed band size: 150,75 kDa why is the actual band size different from the predicted?


      Exposure time: 70 seconds


      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-TrkA antibody [EPR25641-50] (ab302524)
      Immunocytochemistry/ Immunofluorescence - Anti-TrkA antibody [EPR25641-50] (ab302524)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TF-1 (human Erythroleukemia erythroblast) cells labeling TrkA with AB302524 at 1/50 (10.82 µ/mL) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (2 µ/mL) (Green). Confocal image showing membranous and cytoplasmic staining in TF-1 cell line Negative control: 293T (PMID: 10523865). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5µ/mL) (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Primary diluent was used instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution (2 µ/mL).

    • Immunoprecipitation - Anti-TrkA antibody [EPR25641-50] (ab302524)
      Immunoprecipitation - Anti-TrkA antibody [EPR25641-50] (ab302524)

      TrkA was immunoprecipitated from 0.35 mg TF-1 (human Erythroleukemia erythroblast), whole cell lysate 10 µg with ab302524 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB302524 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: TF-1 (human Erythroleukemia erythroblast), whole cell lysate 10 µg (Inset)

      Lane 2: AB302524 at 1/30 IP in TF-1 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302524 in TF-1 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Anti-TrkA antibody [EPR25641-50] (AB302524)
      Anti-TrkA antibody [EPR25641-50] (AB302524)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302524 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302524 は論文での使用が確認できていません。

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