Anti-TrkA 抗体 [EPR25641-120] - BSA and Azide free (Capture) (ab302742)
![Sandwich ELISA - Anti-TrkA antibody [EPR25641-120] - BSA and Azide free (Capture) (ab302742)](https://www.abcam.co.jp/ps/products/302/ab302742/Images/ab302742-2-human-trka-antibody-pair-sandwich-elisa-standard-curve-2.jpg "Sandwich ELISA - Anti-TrkA antibody [EPR25641-120] - BSA and Azide free (Capture) (ab302742)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25641-120] to TrkA - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
製品の概要
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製品名
Anti-TrkA antibody [EPR25641-120] - BSA and Azide free (Capture)
TrkA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25641-120] to TrkA - BSA and Azide free (Capture) -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab302742 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TrkA. The recommended pair for sandwich ELISA is:
Capture: ab302742, Human TrkA Capture Antibody (unconjugated)
Detector: ab302744, Human TrkA Detector Antibody (unconjugated)
The reference range value is 9.77-2500 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25641-120 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302742の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25641-50] to TrkA - BSA and Azide free (Detector) (ab302744).
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| 特記事項 |
|---|
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25641-50] to TrkA - BSA and Azide free (Detector) (ab302744). |
ターゲット情報
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機能
Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. -
組織特異性
Isoform TrkA-II is primarily expressed in neuronal cells; isoform TrkA-I is found in non-neuronal tissues. -
関連疾患
Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]. CIPA is characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating behavior, and mental retardation. This rare autosomal recessive disorder is also known as congenital sensory neuropathy with anhidrosis or hereditary sensory and autonomic neuropathy type IV or familial dysautonomia type II.
Defects in NTRK1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving NTRK1 are found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1; a rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1.
Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 protein kinase domain. -
ドメイン
The transmembrane domain mediates interaction with KIDINS220.
The extracellular domain mediates interaction with NGFR. -
翻訳後修飾
Ligand-mediated auto-phosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. -
細胞内局在
Cell membrane. Endocytosed to the endosomes upon treatment of cells with NGF. - Information by UniProt
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参照データベース
- Entrez Gene: 4914 Human
- Omim: 191315 Human
- SwissProt: P04629 Human
- Unigene: 406293 Human
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別名
- High affinity nerve growth factor receptor antibody
- MTC antibody
- Neurotrophic tyrosine kinase receptor type 1 antibody
see all
画像
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Sandwich ELISA - Anti-TrkA antibody [EPR25641-120] - BSA and Azide free (Capture) (ab302742)Sandwich ELISA of ab302741 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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![Anti-TrkA antibody [EPR25641-120] (BSA and Azide free (Capture)) (AB302742)](https://www.abcam.co.jp/ps/products/302/ab302742/Images/AB302742-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-TrkA antibody [EPR25641-120] (BSA and Azide free (Capture)) (AB302742)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab302742 は論文での使用が確認できていません。
