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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-TRK fused gene 抗体 [EPR8766] (ab156866)

Submit a review Submit a question References (6)
Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Immunoprecipitation - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • OI-RD Scanning - Anti-TRK fused gene antibody [EPR8766] (ab156866)
  • Anti-TRK fused gene antibody [EPR8766] (ab156866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8766] to TRK fused gene
  • Suitable for: WB, ICC/IF, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-TRK fused gene antibody [EPR8766]
    TRK fused gene 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8766] to TRK fused gene

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IHC-P, IPmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal brain tissue lysate and HeLa, A549, 293T, and wild-type HAP1 cell lysates. IHC-P: Human colon and thyroid carcinoma tissues. ICC/IF: HeLa and MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab156866の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
ICC/IF
1/1000.

For unpurified use at 1/100 - 1/250
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
1/10 - 1/100.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 43 kDa.
ICC/IF
1/1000.

For unpurified use at 1/100 - 1/250
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP
1/10 - 1/100.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 組織特異性

    Ubiquitous.

  • 関連疾患

    Defects in TFG are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TFG is found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with NTRK1. The TFG sequence is fused to the 3'-end of NTRK1 generating the TRKT3 (TRK-T3) fusion transcript.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ36137 antibody
      • HMSNP antibody
      • OTTHUMP00000214045 antibody
      • OTTHUMP00000214046 antibody
      • OTTHUMP00000214047 antibody
      • OTTHUMP00000214048 antibody
      • Protein TFG antibody
      • SPG57 antibody
      • TF6 antibody
      • TFG antibody
      • TFG_HUMAN antibody
      • TRK fused antibody
      • TRK fused gene antibody
      • TRK fused gene protein antibody
      • TRK-fused gene protein antibody
      • TRKT3 antibody
      • TRKT3 oncogene antibody
      see all

    画像

    • Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : TFG knockout HeLa cell lysate
      Lane 3 : HAP-1 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 43 kDa
      Observed band size: 57 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab156866 Anti-TRK fused gene antibody [EPR8766] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used.  Wild-type and TFG knockout samples were subjected to SDS-PAGE.  ab156866 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : TFG knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 57 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab156866 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab156866 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)

      Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling TRK fused gene with purified ab156866 at 1/1000. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). 

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control. 

    • Immunoprecipitation - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Immunoprecipitation - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Immunoprecipitaion analysis in 293T cell lysate of TRK fused gene immunoprecipitated with ab156866 at 1/10.

      Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution + Immunoprecipitation pellets from 293T cell lysate at 10 µg
    • Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Immunofluorescent analysis of HeLa cells labeling TRF fused gene with ab156866 at 1/100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TRF fused gene with ab156966 at 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)

      Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling TRF fused gene with ab156866 at 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      OI-RD Scanning - Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-TRK fused gene antibody [EPR8766] (ab156866)
      Anti-TRK fused gene antibody [EPR8766] (ab156866)

    参考文献 (6)

    ab156866 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab156866 は 6 報の論文で使用されています。

    • Shi JH  et al. TRK-fused gene (TFG) regulates ULK1 stability via TRAF3-mediated ubiquitination and protects macrophages from LPS-induced pyroptosis. Cell Death Dis 13:93 (2022). PubMed: 35091545
    • Yamamotoya T  et al. Involvement of neuronal and muscular Trk-fused gene (TFG) defects in the development of neurodegenerative diseases. Sci Rep 12:1966 (2022). PubMed: 35121777
    • Inukai R  et al. The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B. Int J Mol Sci 22:N/A (2021). PubMed: 33503978
    • Carinci M  et al. TFG binds LC3C to regulate ULK1 localization and autophagosome formation. EMBO J 40:e103563 (2021). PubMed: 33932238
    • Yamamotoya T  et al. Trk-fused gene (TFG) regulates pancreatic ß cell mass and insulin secretory activity. Sci Rep 7:13026 (2017). PubMed: 29026155
    • Kanadome T  et al. The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein. FEBS J 284:56-76 (2017). PubMed: 27813252

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