Anti-TRIP6 抗体 (ab70747)
Key features and details
- Rabbit polyclonal to TRIP6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRIP6 antibody
TRIP6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRIP6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 1 and 50 of human TRIP6 (NP_003293.1)
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ポジティブ･コントロール
- Whole cell lysate from HeLa or 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70747の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor. -
組織特異性
Abundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors. -
配列類似性
Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains. -
ドメイン
The LIM zinc-binding domains mediate interaction with LPAR2 and with S.typhimurium protein sseI. -
翻訳後修飾
Phosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13. -
細胞内局在
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7205 Human
- Omim: 602933 Human
- SwissProt: Q15654 Human
- Unigene: 534360 Human
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別名
- MGC10556 antibody
- MGC10558 antibody
- MGC29959 antibody
see all
画像
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Western blot - Anti-TRIP6 antibody (ab70747)All lanes : Anti-TRIP6 antibody (ab70747) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation - Anti-TRIP6 antibody (ab70747)1mg whole cell lysate from HeLa cells was immunprecipitated using ab70747 at 3µg/mg of lysate. For the subsequent western blot, 20% of the immunoprecipitate was probed with ab70747 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70747 は 3 報の論文で使用されています。
- Venkatramanan S et al. TRIP6 is required for tension at adherens junctions. J Cell Sci 134:N/A (2021). PubMed: 33558314
- Liu W et al. TRIP6 regulates the proliferation, migration, invasion and apoptosis of osteosarcoma cells by activating the NF-?B signaling pathway. Exp Ther Med 19:2317-2325 (2020). PubMed: 32104300
- Gou H et al. TTPAL Promotes Colorectal Tumorigenesis by Stabilizing TRIP6 to Activate Wnt/ß-Catenin Signaling. Cancer Res 79:3332-3346 (2019). PubMed: 31018940