AB70747

Anti-TRIP6 抗体

Name: Anti-TRIP6 抗体 (ab70747) | Abcam
Brand: Abcam Limited
SKU: AB70747
Price: 75000 JPY
Availability: InStock

Anti-TRIP6 antibody

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(3 Publications)

Rabbit Polyclonal TRIP6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRIP6.

別名を表示する

OIP1, TRIP6, Thyroid receptor-interacting protein 6, TR-interacting protein 6, TRIP-6, Opa-interacting protein 1, Zyxin-related protein 1, OIP-1, ZRP-1

2 Images

Unknown

Immunoprecipitation - Anti-TRIP6 antibody (AB70747)

1mg whole cell lysate from HeLa cells was immunprecipitated using ab70747 at 3µg/mg of lysate. For the subsequent western blot, 20% of the immunoprecipitate was probed with ab70747 at 1ug/ml. Detection : chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TRIP6 antibody (ab70747)

Predicted band size: 50 kDa

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Unknown

Western blot - Anti-TRIP6 antibody (AB70747)

All lanes:

Western blot - Anti-TRIP6 antibody (ab70747) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 50 kDa

Observed band size: 55 kDa

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Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human TRIP6. The exact immunogen used to generate this antibody is proprietary information.

Q15654

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRIP6 also known as Thyroid Hormone Receptor Interactor 6 or ZRP-1 (Zyxin Related Protein 1) is a cytoskeletal adaptor protein with a molecular mass of approximately 55 kDa. Its primary function involves acting as a scaffold protein that facilitates interactions between several intracellular signaling molecules. TRIP6 localizes mainly to focal adhesion sites and the actin cytoskeleton where it regulates cell motility and organization. This protein is usually expressed in various tissues but expression levels are notably prominent in the lymphoid tissues and the gastrointestinal tract.

Biological function summary

The mechanical interactions of TRIP6 influence cell migration adhesion and morphogenesis. It functions as a component of the focal adhesion complex where it plays an important role in linking the actin cytoskeleton to integrin receptors. Additionally TRIP6 participates in the regulation of gene expression by translocating to the nucleus and interacting with transcription factors to modulate specific gene targets.

Pathways

The involvement of TRIP6 is significant within signaling pathways such as the RhoA pathway and the NF-kB signaling pathway. Through RhoA TRIP6 contributes to the modulation of cell shape and motility impacting cellular processes such as angiogenesis and wound healing. In the NF-kB pathway TRIP6 can influence inflammatory responses by affecting gene transcription. TRIP6 is associated with proteins like paxillin and focal adhesion kinase (FAK) which are key components in these pathways.

TRIP6 has notable implications in cancer progression and inflammatory diseases. It has been implicated as a contributor to tumorigenesis due to its role in cell motility and invasion often showing altered expression in various cancers such as breast and prostate cancer. TRIP6 also interacts with the protein zyxin and this interaction influences cancer cell metastasis. Additionally TRIP6 can affect inflammatory responses in disorders such as inflammatory bowel disease where its modulation impacts cellular pathways related to inflammation and immune response.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.

See full target information TRIP6

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science : PubMed33558314

2021

TRIP6 is required for tension at adherens junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Srividya Venkatramanan,Consuelo Ibar,Kenneth D Irvine

Experimental and therapeutic medicine 19:2317-2325 PubMed32104300

2020

TRIP6 regulates the proliferation, migration, invasion and apoptosis of osteosarcoma cells by activating the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liu,Li Cheng,Qingning Li,Juehua Jing

Cancer research 79:3332-3346 PubMed31018940

2019

TTPAL Promotes Colorectal Tumorigenesis by Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Gou,Jessie Qiaoyi Liang,Lijing Zhang,Huarong Chen,Yanquan Zhang,Rui Li,Xiaohong Wang,Jiafu Ji,Joanna H Tong,Ka-Fai To,Joseph J Y Sung,Francis K L Chan,Jing-Yuan Fang,Jun Yu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-TRIP6 抗体

Anti-TRIP6 antibody

Immunoprecipitation - Anti-TRIP6 antibody (AB70747)

Western blot - Anti-TRIP6 antibody (AB70747)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise