Anti-Triosephosphate isomerase 抗体 (ab28760)
Key features and details
- Goat polyclonal to Triosephosphate isomerase
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Dog, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Triosephosphate isomerase antibody
Triosephosphate isomerase 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Triosephosphate isomerase -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms. -
アプリケーション
適用あり: Flow Cyt, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Dog, Human
交差が予測される動物種: Rat, Cow -
免疫原
Synthetic peptide:
C-LKPE FVDIINAKQ
, corresponding to C terminal amino acids 237-249 of Human Triosephosphate isomerase, according to NP_000356. -
ポジティブ・コントロール
- WB: HEK293, HeLa, Jurkat, HepG2, RAW264.7, KNRK and MDCK cell lysates. IHC-P: Human liver tissue. Flow Cyt: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28760の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 10 µg/ml.
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WB | (2) |
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. -
細胞内局在
Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space. -
参照データベース
- Entrez Gene: 281543 Cow
- Entrez Gene: 477711 Dog
- Entrez Gene: 7167 Human
- Entrez Gene: 21991 Mouse
- Entrez Gene: 24849 Rat
- Omim: 190450 Human
- SwissProt: Q5E956 Cow
- SwissProt: P54714 Dog
see all -
別名
- epididymis secretory protein Li 49 antibody
- HEL-S-49 antibody
- MGC88108 antibody
see all
画像
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All lanes : Anti-Triosephosphate isomerase antibody (ab28760) at 0.001 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 26 kDaDetected by chemiluminescence.
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Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triosephosphate isomerase antibody (ab28760)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, staining Triosephosphate isomerase with ab28760 at 2 µg/mL. Antigen retrieval was performed by heat mediation in a Tris/EDTA buffer (pH 9).
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All lanes : Anti-Triosephosphate isomerase antibody (ab28760) at 0.001 µg/ml
Lane 1 : RAW264.7 cell lysate
Lane 2 : KNRK cell lysate
Lane 3 : MDCK cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 26 kDaDetected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (12)
ab28760 は 12 報の論文で使用されています。
- Yin GQ et al. Differential proteomic analysis of children infected with respiratory syncytial virus. Braz J Med Biol Res 54:e9850 (2021). PubMed: 33656056
- Daniel B et al. Endothelial Cell-Derived Triosephosphate Isomerase Attenuates Insulin Secretion From Pancreatic Beta Cells of Male Rats. Endocrinology 162:N/A (2021). PubMed: 33341896
- Rong X et al. Chronic Periodontitis and Alzheimer Disease: A Putative Link of Serum Proteins Identification by 2D-DIGE Proteomics. Front Aging Neurosci 12:248 (2020). PubMed: 32973486
- Nagashima D et al. Proteomic analysis of hippocampal proteins in acrylamide-exposed Wistar rats. Arch Toxicol 93:1993-2006 (2019). PubMed: 31123803
- Zhang CS et al. Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Nature 548:112-116 (2017). PubMed: 28723898