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リコンビナントRabMAb

Anti-TRIM9 抗体 [EPR25103-71] - BSA and Azide free (ab300516)

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Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25103-71] to TRIM9 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free
    TRIM9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25103-71] to TRIM9 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WB, IHC-Pmore details
    適用なし: Flow Cyt (Intra) or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human cerebellum, IMR-32, SK-N-BE, Y79; Mouse: brain, hippocampus, cerebellum tissue lysates. Rat: cerebellum, and brain tissue lysates. IHC-P: Mouse cerebrum. IP: SK-N-BE and Mouse brain cells.

  • 特記事項

    ab300516 is the carrier-free version of ab300515.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300516の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

ターゲット情報

  • 関連性

    TRIM9 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.

  • 細胞内局在

    Cytoplasmic

  • 参照データベース

    • 別名

      • E3 ubiquitin-protein ligase TRIM9 antibody
      • HGNC:16288 antibody
      • Homolog of rat RING finger Spring antibody
      • KIAA0282 antibody
      • RING finger protein 91 antibody
      • RING type E3 ubiquitin transferase TRIM9 antibody
      • RNF 91 antibody
      • RNF91 antibody
      • SPRING antibody
      • TRIM 9 antibody
      • Tripartite motif containing 9 antibody
      • Tripartite motif containing protein 9 antibody
      • Tripartite motif protein 9 antibody
      see all

    画像

    • Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      All lanes : Anti-TRIM9 antibody [EPR25103-71] (ab300515) at 1/1000 dilution

      Lane 1 : Human cerebellum tissue lysate at 20 µg
      Lane 2 : Human liver tissue lysate at 40 µg
      Lane 3 : IMR-32 (human neuroblastoma neuroblast), whole cell lysate at 20 µg
      Lane 4 : SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg
      Lane 5 : Y79 (human retinoblastoma retinoblastoma), whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 79, 61 kDa why is the actual band size different from the predicted?



      This data was developed using ab300515, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

      Negative control: human liver tissue (PMID:20085810).


      The antibody detects isoform of TRIM9 around 61kDa which consistent with what has been described in the literature (PMID:20085810). The identity of the lower MW band at approximately 37 kDa (in lane 1, 2) is unknown.


      The blot of lane 5 was developed using a high sensitivity ECL substrate.

      Exposure time: Lane 1-4: 136 seconds Lane 5: 3 minutes 

    • Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      All lanes : Anti-TRIM9 antibody [EPR25103-71] (ab300515) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate at 20 µg
      Lane 2 : Mouse hippocampus tissue lysate at 20 µg
      Lane 3 : Mouse liver tissue lysate at 40 µg
      Lane 4 : Mouse cerebellum tissue lysate at 20 µg
      Lane 5 : Rat cerebellum tissue lysate at 20 µg
      Lane 6 : Rat brain tissue lysate at 20 µg
      Lane 7 : Rat liver tissue lysate at 40 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution


      This data was developed using ab300515, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The identity of the bands between 37 kDa and 25 kDa are unknown.

      Exposure time: Lane 1-5: 81 secomds Lane 6, 7: 52 minutes

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

      This data was developed using ab300515, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRIM9 with ab300515 at 1/5000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Positive staining on mouse cerebrum is observed (PMID: 20085810). The section was incubated with ab300515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

      This data was developed using ab300515, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TRIM9 with ab300515 at 1/5000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Negative control: no staining on mouse liver is observed (PMID: 20085810). The section was incubated with ab300515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

      This data was developed using ab300515, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling TRIM9 with ab300515 at 1/5000 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection kit). Negative control: no staining on mouse spleen is observed (PMID: 20085810). The section was incubated with ab300515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by a ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

      This data was developed using ab300515, the same antibody clone in a different buffer formulation.

      TRIM9 was immunoprecipitated from 0.35 mg SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate 10 μg with ab300515 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300515 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate 10 μg (Input)

      Lane 2: ab300515 IP in SK-N-BE(2) whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300515 in SK-N-BE(2) whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Observed MW (kDa): 79, 61. 

      Exposure time: 23 seconds. 

    • Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

      This data was developed using ab300515, the same antibody clone in a different buffer formulation.

      TRIM9 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 μg with ab300515 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300515 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: mouse brain tissue lysate 10 μg (Input)

      Lane 2: ab300515 IP in mouse brain tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300516 in mouse brain tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Observed MW (kDa): 79, 61. 

      Exposure time: 41 seconds 

    • Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
      Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300516 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300516 は論文での使用が確認できていません。

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