Anti-TRIM59 抗体 (ab69639)
Key features and details
- Rabbit polyclonal to TRIM59
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRIM59 antibody -
製品の詳細
Rabbit polyclonal to TRIM59 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthesized peptide derived from internal region of human TRIM59.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69639の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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配列類似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 RING-type zinc finger. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 286827 Human
- Entrez Gene: 66949 Mouse
- SwissProt: Q8IWR1 Human
- SwissProt: Q922Y2 Mouse
- Unigene: 212957 Human
- Unigene: 490341 Mouse
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別名
- 2310035M22Rik antibody
- 2700022F13Rik antibody
- MGC129860 antibody
see all
画像
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Western blot - Anti-TRIM59 antibody (ab69639)All lanes : Anti-TRIM59 antibody (ab69639) at 1/500 dilution
Lane 1 : extracts from NIH-3T3 cells
Lane 2 : extracts from NIH-3T3 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TRIM59 antibody (ab69639)This image shows HeLa cells stained with ab69639 at 1/500 dilution, with and without the immunising peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM59 antibody (ab69639)This image shows human brain tissue stained with ab69639 at 1/50 dilution, with and without the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab69639 は 10 報の論文で使用されています。
- Cai H et al. SARS-CoV-2 viral protein ORF3A injures renal tubules by interacting with TRIM59 to induce STAT3 activation. Mol Ther 31:774-787 (2023). PubMed: 36523164
- Wang T et al. Circ_0006324 regulates cell proliferation, cell-cycle progression, apoptosis, and glycolysis of non-small cell lung cancer cells through miR-496/TRIM59 axis. J Biochem Mol Toxicol 37:e23473 (2023). PubMed: 37545326
- Liu G et al. Tripartite motif containing 59 (TRIM59) promotes esophageal cancer progression via promoting MST4 expression and ERK pathway. J Recept Signal Transduct Res N/A:1-8 (2020). PubMed: 32340525
- Armenta-Castro E et al. Histone H3K9 and H3K14 acetylation at the promoter of the LGALS9 gene is associated with mRNA levels in cervical cancer cells. FEBS Open Bio 10:2305-2315 (2020). PubMed: 32902187
- Gong LP et al. Epstein-Barr virus-derived circular RNA LMP2A induces stemness in EBV-associated gastric cancer. EMBO Rep 21:e49689 (2020). PubMed: 32790025
- Sang Y et al. CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. Nat Commun 10:4013 (2019). PubMed: 31488827
- Sang Y et al. TRIM59 Promotes Gliomagenesis by Inhibiting TC45 Dephosphorylation of STAT3. Cancer Res 78:1792-1804 (2018). PubMed: 29386185
- Su X et al. Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development. Cell Death Dis 9:302 (2018). PubMed: 29467473
- Tan P et al. TRIM59 promotes breast cancer motility by suppressing p62-selective autophagic degradation of PDCD10. PLoS Biol 16:e3000051 (2018). PubMed: 30408026
- Wu W et al. Knockdown of tripartite motif-59 inhibits the malignant processes in human colorectal cancer cells. Oncol Rep 38:2480-2488 (2017). PubMed: 28849218