AB154821

Anti-TRIM56 抗体 [EPR10582]

Anti-TRIM56 antibody [EPR10582]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal TRIM56 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

別名を表示する

RNF109, TRIM56, E3 ubiquitin-protein ligase TRIM56, RING finger protein 109, Tripartite motif-containing protein 56

8 Images

Flow Cytometry (Intracellular) - Anti-TRIM56 antibody [EPR10582] (AB154821)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-TRIM56 antibody [EPR10582] (AB154821)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling TRIM56 with purified ab154821 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-TRIM56 antibody [EPR10582] (AB154821)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TRIM56 antibody [EPR10582] (AB154821)

ab154821 staining TRIM56 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab154821 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1 : Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2 : Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

Lab

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : TRIM56 knockout HAP1 cell lysate (20 μg)
Lane 3 : MCF7 cell lysate (20 μg)
Lane 4 : A375 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab154821 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab154821 was shown to specifically react with TRIM56 when TRIM56 knockout samples were used. Wild-type and TRIM56 knockout samples were subjected to SDS-PAGE. ab154821 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye^® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10582] (ab154821)

Predicted band size: 81 kDa

false

Lab

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Lanes 1-4 : Merged signal (red and green). Green - ab154821 observed at 88 kDa. Red - loading control ab8245 observed at 36 kDa.

ab154821 Anti-TRIM56 antibody [EPR10582] was shown to specifically react with TRIM56 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267063 (knockout cell lysate ab258250) was used. Wild-type and TRIM56 knockout samples were subjected to SDS-PAGE. ab154821 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TRIM56 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TRIM56 knockout A549 cell line (<a href='/products/cell-lines/human-trim56-knockout-a549-cell-line-ab267063'>ab267063</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 88 kDa

false

Lab

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Lanes 1-4 : Merged signal (red and green). Green - ab154821 observed at 88 kDa. Red - loading control ab8245 observed at 36 kDa.

ab154821 Anti-TRIM56 antibody [EPR10582] was shown to specifically react with TRIM56 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267062 (knockout cell lysate ab258249) was used. Wild-type and TRIM56 knockout samples were subjected to SDS-PAGE. ab154821 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TRIM56 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TRIM56 knockout A549 cell line (<a href='/products/cell-lines/human-trim56-knockout-a549-cell-line-ab267062'>ab267062</a>)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 88 kDa

false

Unknown

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/1000 dilution

Lane 1:

MCF 7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A375 cell lysate at 10 µg

Predicted band size: 81 kDa

false

Lab

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/5000 dilution

All lanes:

A549 (human lung carcinoma) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 81 kDa

false

Lab

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

All lanes:

Western blot - Anti-TRIM56 antibody [EPR10582] (ab154821) at 1/1000 dilution

All lanes:

MCF-7 (human breast carcinoma) whole cell lysates at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 81 kDa

Observed band size: 81 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10582

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRIM56 also known as Tripartite Motif Containing 56 or RNF109 is a protein involved in various cellular mechanisms. It weighs around 79 kDa and is expressed in many tissues including the spleen lymph nodes and lungs. TRIM56 contains a RING finger domain B-box motifs and a coiled-coil region which are typical features of the TRIM protein family. These domains suggest roles in ubiquitination and protein-protein interactions which are essential for its functions in signaling and regulatory processes.

Biological function summary

TRIM56 participates in immune regulation and antiviral defense. It acts as an interferon-stimulated gene product and does not appear to be part of a larger protein complex. Instead it induces antiviral states by mediating the innate immune response to viral infections. TRIM56 enhances the production of type I interferons by modifying the activation pathways. This function places TRIM56 as an immune defense agent which can restrict the replication of various viruses such as flaviviruses and coronaviruses therefore harboring significant implications in pathogen defense responses.

Pathways

TRIM56 integrates into the antiviral and innate immunity pathways. It prominently situates within the interferon signaling pathway enhancing the body's defense through the upregulation of interferon production. TRIM56 interacts with several proteins in these pathways including STING and MAVS which are known to be important mediators in the recognition of viral presence. Through these interactions TRIM56 facilitates the activation of downstream signaling cascades leading to the expression of interferon-stimulated genes.

TRIM56 exhibits significant connections to viral infections and autoimmune diseases. Its role in antiviral responses implicates it in diseases like hepatitis C as it can suppress viral replication. Although TRIM56 aids in defending against infections its dysregulation may also link to autoimmune conditions where its interaction with proteins such as IFNAR1 can result in excessive immune responses leading to tissue damage and inflammation. Such dual roles highlight TRIM56 as an important regulator in both protective and pathological immune responses.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity by mediating ubiquitination of CGAS and STING1 (PubMed : 21289118, PubMed : 29426904). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets STING1 to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Also mediate monoubiquitination of CGAS, thereby promoting CGAS oligomerization and subsequent activation (PubMed : 29426904). Promotes also TNFalpha-induced NF-kappa-B signaling by mediating 'Lys-63'-linked ubiquitination TAK1, leading to enhanced interaction between TAK1 and CHUK/IKKalpha (PubMed : 35952808). Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES (PubMed : 22948160). Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed : 22948160). Acts as a restriction factor of Zika virus through direct interaction with the viral RNA via its C-terminal region (PubMed : 31251739).

See full target information TRIM56

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed40242951

2025

Preliminary exploration of endoplasmic reticulum stress transmission in astrocytes and neurons, and its mediators.

Applications

Unspecified application

Species

Unspecified reactive species

Yating Ling,Muhammad Abid Hayat,Xiaorui Lv,Dongdong Niu,Yu Zeng,Yun Qiu,Bo Chen,Jiabo Hu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM56 抗体 [EPR10582]

Anti-TRIM56 antibody [EPR10582]

Flow Cytometry (Intracellular) - Anti-TRIM56 antibody [EPR10582] (AB154821)

Immunocytochemistry/ Immunofluorescence - Anti-TRIM56 antibody [EPR10582] (AB154821)

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

Western blot - Anti-TRIM56 antibody [EPR10582] (AB154821)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise