Anti-TRIM33 抗体 [EPR25102-19] (BSA and Azide free) (ab300147)
![Western blot - Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free) (AB300147)](https://www.abcam.co.jp/ps/products/300/ab300147/Images/AB300147-485726-300146WB1.jpg "Western blot - Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free) (AB300147)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25102-19] to TRIM33 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free)
TRIM33 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25102-19] to TRIM33 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: ChIP,Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Wild-type HAP1 whole cell lysate, K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate, 293T (human embryonic kidney epithelial cell) whole cell lysate. IP: K-562 whole cell lysate.
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特記事項
Ab300147 is a carrier free version of ab300146.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25102-19 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. |
ターゲット情報
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機能
Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade). -
組織特異性
Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TRIM33 is found in thyroid papillary carcinomas. Translocation t(1;10)(p13;q11) with RET. The translocation generates the TRIM33/RET (PTC7) oncogene. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
細胞内局在
Nucleus. In discrete nuclear dots resembling nuclear bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 51592 Human
- Omim: 605769 Human
- SwissProt: Q9UPN9 Human
- Unigene: 26837 Human
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別名
- 8030451N04Rik antibody
- AI413936 antibody
- cb1085 antibody
see all
画像
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Western blot - Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free) (AB300147)All lanes : Anti-TRIM33 antibody [EPR25102-19] (ab300146) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TRIM33 knockout HAP1 whole cell lysate
Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/100000 dilution
Predicted band size: 122 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab300146, the same antibody clone in a different buffer formulation.
Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS was used as a blocking and diluting buffer and concentration.
Lanes 1-4: Merged signal (red and green). Green - ab300146 observed at 150 kDa. Red - loading control ab8245 observed at 36 kDa.
ab300146 Anti-TRIM33 antibody [EPR25102-19] was shown to specifically react with TRIM33 in wild-type HAP1 cells. Loss of signal was observed when knockout cell line was used. Wild-type and TRIM33 knockout samples were subjected to SDS-PAGE. ab300146 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging. -
![Immunoprecipitation - Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free) (AB300147)](https://www.abcam.co.jp/ps/products/300/ab300147/Images/AB300147-4-anti-trim33-antibody-epr2510219-immunoprecipitation-k562.jpg)
Immunoprecipitation - Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free) (AB300147)This data was developed using ab300146, the same antibody clone in a different buffer formulation.
TRIM33 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg with ab300146 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300146 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
Lane 2: K-562 whole cell lysate
Lane 3: Rabit monoclonal IgG (ab172730) instead of ab300146 in K-562 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
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![Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free) (AB300147)](https://www.abcam.co.jp/ps/products/300/ab300147/Images/AB300147-485678-Benefits-of-Recombinant-antibodies.jpg)
Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free) (AB300147)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300147 は論文での使用が確認できていません。
