Anti-TRIM32 抗体 [EP6355]

• WB

Supplier Data

Western blot - Anti-TRIM32 antibody [EP6355] (AB131223)

The bands above 75kDa are ubiquitination bands which was described in the literature (PMID : 30884854, 16243356, 21628460). Blocking buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TRIM32 antibody [EP6355] (ab131223) at 1/2000 dilution

All lanes:

Human skeletal muscle lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 73 kDa

false

Exposure time: 60s

• WB

Supplier Data

Western blot - Anti-TRIM32 antibody [EP6355] (AB131223)

Although some papers support the expression of TRIM32 in SK-BR-3, T-47D, MCF7, MDA-MB-435S, MD-MB-468 (PMID : 29721043), ab131223 can’t detect monomer TRIM32 band of interest in these cell lines. The bands above 75kDa are ubiquitination bands which was described in the literature (PMID : 30884854, 16243356, 21628460). Blocking buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1 & Lane 2 : 3 seconds; Lane 3- Lane7 : 180 seconds.

All lanes:

Western blot - Anti-TRIM32 antibody [EP6355] (ab131223) at 1/200 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with TRIM32 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysate at 20 µg

Lane 7:

Human skeletal muscle lysate at 20 µg

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TRIM32 antibody [EP6355] (AB131223)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.