AB167154

Anti-TRIM25/EFP 抗体 [EPR7315]

Name: Anti-TRIM25/EFP 抗体 [EPR7315] (ab167154) | Abcam
Brand: Abcam Limited
SKU: AB167154
Price: 53000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-TRIM25/EFP antibody [EPR7315]

RabMAb
Recombinant
KO Validated
詳細を見る

(1 Review)

(39 Publications)

Anti-TRIM25/EFP antibody [EPR7315] (ab167154) is a rabbit monoclonal antibody detecting TRIM25/EFP in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

別名を表示する

EFP, RNF147, ZNF147, TRIM25, E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, RING-type E3 ubiquitin transferase, RING-type E3 ubiquitin transferase TRIM25, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

ab167154 staining TRIM25/EFP in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

ab167154 staining TRIM25/EFP in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a tubulin counterstain at a dilution of 1/200 and DAPI was used as a nuclear counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TRIM25/EFP with ab167154 at 1/100 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Immunofluorescent analysis of HeLa cells labeling TRIM25/EFP with ab167154 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling TRIM25/EFP with purified ab167154 at 1/90 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Lab

Immunoprecipitation - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

ab167154 immunoprecipitating TRIM25/EFP. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/40 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate (10ug)
Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab167154 in HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate.

All lanes:

Immunoprecipitation - Anti-TRIM25/EFP antibody [EPR7315] (ab167154)

Predicted band size: 71 kDa

Observed band size: 71 kDa

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Unknown

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

All lanes:

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (ab167154) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 71 kDa

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Lab

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (ab167154) at 1/50000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

false

Lab

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Lanes 1 - 4 : Merged signal (red and green). Green - ab167154 observed at 71 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab167154 was shown to specifically react with TRIM25/EFP in wild-type HAP1 cells as signal was lost in TRIM25/EFP knockout cells. Wild-type and TRIM25/EFP knockout samples were subjected to SDS-PAGE. ab167154 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (ab167154) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TRIM25/EFP knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

Predicted band size: 71 kDa

false

Lab

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (ab167154) at 1/10000 dilution

All lanes:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Lab

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Blocking and diluting buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (ab167154) at 1/2000 dilution

All lanes:

SP2/0 (mouse spleen) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

CiteAb

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

TRIM25/EFP western blot using anti-TRIM25/EFP antibody [EPR7315] ab167154. Publication image and figure legend from Lang, F., Aravamudhan, S., et al., 2017, Dis Model Mech, PubMed 28546288.

ab167154 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab167154 please see the product overview.

Selected proteins associated with proteolysis and time-dependent protein changes in sarcomeric proteins. (A-E) Bar diagrams showing protein ratios of selected E2-ubiqutin conjugation enzymes (A), E3-ubiquitin ligases (B), tripartite motif family (MURFs; C), deubiquitinating enzymes (D) and autophagy marker proteins (E) during the time course after denervation. (F) Schematic representation of the sarcomere; proteins labelled blue are upregulated and proteins labelled red are downregulated. (G-J) Protein expression time profiles of selected differentially expressed myofibrillar proteins. Log2 SILAC ratios were plotted against time [from day 1 to 14 after denervation (t1=day 1; t2=day 4; t3=day 7; t4=day 14)]. (K) Immunoblot of lysates from control and denervated GAST using antibodies for TRIM25, AKT2, USP14 and tubulin. Dystrophin was used as a loading control. *Significant regulation (FDR cut-off=0.05). **Direct heavy/light SILAC ratio detected only in denervated muscle.

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR7315

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60 - 1/90", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-TRIM25/EFP antibody [EPR7315] (ab167154) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of TRIM25/EFP?
Anti-TRIM25/EFP [EPR7315] (ab167154) specifically detects a band for TRIM25/EFP (UniProt: Q14258) at a molecular weight of 71kDa.

Trusted by the scientific community
Anti-TRIM25/EFP [EPR7315] (ab167154) was first used in a scientific publication in 2013 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-TRIM25/EFP antibody [EPR7315] (ab167154) has been confirmed by Western blot testing in TRIM25 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR7315] also available for your convenience: ab167154, Alexa Fluor® 488 - ab200781, HRP - ab200788, Carrier free - ab232356

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRIM25 also known as EFP is a protein involved in antiviral response mechanisms. TRIM25 has a molecular mass of approximately 70 kDa and is part of the TRIM (Tripartite Motif) family which contains three distinct zinc-binding domains followed by a coiled-coil region. This protein exhibits strong expression in a variety of tissues including lung liver and intestines. As a member of the E3 ubiquitin ligase enzyme family TRIM25 plays a direct role in protein ubiquitination which tags specific proteins for degradation by the proteasome.

Biological function summary

TRIM25 functions as a significant modulator of the innate immune response against viral infections. In cells it acts within the RIG-I signaling complex directly promoting the RIG-I-MAVS pathway that detects viral RNA. TRIM25 mediates the ubiquitination of RIG-I allowing for its proper activation and subsequent signal transduction facilitating a robust antiviral state. TRIM25's role extends beyond antiviral defenses to include regulatory functions in cell proliferation and differentiation.

Pathways

TRIM25 engages actively in the innate immune signaling and JAK-STAT pathways. It interacts and cooperates with RIG-I in the recognition and response to viral tRNA leading to the production of interferons that establish an antiviral environment. Furthermore TRIM25 also connects with proteins like TRIM21 which further engages the immune response. This interaction enhances the overall ability of cells to fend off viral pathogens efficiently.

Altered TRIM25 function is associated with conditions like systemic lupus erythematosus (SLE) and various cancers. In the context of SLE TRIM25's regulatory role in innate immunity can affect the severity and progression of this autoimmune disorder. Additionally in some cancers abnormal TRIM25 expression can influence cell cycle regulation and metastasis. Through its ubiquitination activities TRIM25 interacts directly with proteins such as p53 affecting tumor suppression pathways and contributing to the complexity of disease development and progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed : 16352599). Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1 (PubMed : 17392790, PubMed : 29357390, PubMed : 30193849, PubMed : 31710640, PubMed : 33849980, PubMed : 36045682). Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed : 17392790, PubMed : 23950712). Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed : 30193849). Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed : 16352599, PubMed : 17069755). Mediates estrogen action in various target organs (PubMed : 22452784). Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed : 22452784). Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity). Plays an essential role in the antiviral activity of ZAP/ZC3HAV1; an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA (PubMed : 28060952, PubMed : 28202764). Also mediates the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability (PubMed : 36067236).

See full target information TRIM25

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文献 (39)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:32734 PubMed40993169

2025

Diesel particulate matter-induced proteomic changes in three-dimensional spheroids derived from human primary cells of various tissue origins.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Jin Cho,Hae Dong Jeong,Young June Jeon,Soobin Choi,Ji Hyun Back,Ji Hun Wi,Yae Eun Park,Seung-Hee Gwak,Mi Jung Ji,Hyun-Mee Park,Hyuk Jeong,So Yeon Kim,Ji Eun Lee

Journal of virology 99:e0089825 PubMed40719442

2025

The vaccinia virus protein, C16, promotes the ubiquitylation and relocalization of the antiviral E3 ubiquitin-ligase, TRIM25.

Applications

Unspecified application

Species

Unspecified reactive species

Jianing Dong,Shu Yue Luo,Summer Smyth,Grace Melvie,Olivier Julien,Robert J Ingham

International journal of biological sciences 21:874-892 PubMed39781456

2025

Cold atmospheric plasma potentiates ferroptosis via EGFR(Y1068)-mediated dual axes on GPX4 among triple negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Dai,Ziyao Xu,Xinyu Lv,Chao Li,Ruichen Jiang,Danjun Wang,Ming Xi,Tian Li

Molecular medicine (Cambridge, Mass.) 30:220 PubMed39563244

2024

BMSC-derived exosomes promote osteoporosis alleviation via M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbin Zhang,Jing Bai,Bin Xiao,Chunyan Li

Veterinary research 55:44 PubMed38589930

2024

Swine acute diarrhea syndrome coronavirus nucleocapsid protein antagonizes the IFN response through inhibiting TRIM25 oligomerization and functional activation of RIG-I/TRIM25.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyu Zhang,Hongyan Shi,Liaoyuan Zhang,Tingshuai Feng,Jianfei Chen,Xin Zhang,Zhaoyang Ji,Zhaoyang Jing,Xiaoyuan Zhu,Dakai Liu,Xiaoman Yang,Miaomiao Zeng,Da Shi,Li Feng

Microbiology spectrum 12:e0275823 PubMed38100396

2023

CSFV restricts necroptosis to sustain infection by inducing autophagy/mitophagy-targeted degradation of RIPK3.

Applications

Unspecified application

Species

Unspecified reactive species

Keke Wu,Bingke Li,Xiaoai Zhang,Yiqi Fang,Sen Zeng,Wenshuo Hu,Xiaodi Liu,Xueyi Liu,Zhimin Lu,Xiaowen Li,Wenxian Chen,Yuwei Qin,Bolun Zhou,Linke Zou,Feifan Zhao,Lin Yi,Mingqiu Zhao,Shuangqi Fan,Jinding Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303484 PubMed37946697

2023

Inhibition of CARM1-Mediated Methylation of ACSL4 Promotes Ferroptosis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shengjie Feng,Zejun Rao,Jiakun Zhang,Xiaowei She,Yaqi Chen,Kairui Wan,Haijie Li,Chongchong Zhao,Yongdong Feng,Guihua Wang,Junbo Hu,Xuelai Luo

Cell death & disease 14:525 PubMed37582769

2023

Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Yuxi Ou,Quan Zhou,Yingchun Liang,Weijian Li,Yiling Chen,Wensun Chen,Siqi Wu,Yifan Chen,Xiyu Dai,Xinan Chen,Tian Chen,Shengming Jin,Yufei Liu,Limin Zhang,Shenghua Liu,Yun Hu,Lujia Zou,Shanhua Mao,Haowen Jiang

PLoS pathogens 19:e1011357 PubMed37146066

2023

CpG dinucleotide enrichment in the influenza A virus genome as a live attenuated vaccine development strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Colin P Sharp,Beth H Thompson,Tessa J Nash,Ola Diebold,Rute M Pinto,Luke Thorley,Yao-Tang Lin,Samantha Sives,Helen Wise,Sara Clohisey Hendry,Finn Grey,Lonneke Vervelde,Peter Simmonds,Paul Digard,Eleanor R Gaunt

Journal of virology 97:e0184622 PubMed36916924

2023

Strain-Dependent Restriction of Human Cytomegalovirus by Zinc Finger Antiviral Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Jose Lista,Adam A Witney,Jenna Nichols,Andrew J Davison,Harry Wilson,Katie A Latham,Benjamin J Ravenhill,Katie Nightingale,Richard J Stanton,Michael P Weekes,Stuart J D Neil,Chad M Swanson,Blair L Strang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM25/EFP 抗体 [EPR7315]

Anti-TRIM25/EFP antibody [EPR7315]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Immunocytochemistry/ Immunofluorescence - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Immunocytochemistry/ Immunofluorescence - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Flow Cytometry (Intracellular) - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Immunoprecipitation - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

Western blot - Anti-TRIM25/EFP antibody [EPR7315] (AB167154)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (39)

代替製品

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Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise